A time course study on the "in vitro" effects of T3 and testosterone on androgen and estrogen receptors in peripuberal primary rat Sertoli cells.
A time course study on the "in vitro" effects of T3 and testosterone on androgen and estrogen receptors in peripuberal primary rat Sertoli cells.
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DOI：
10.1055/s-0029-1211755
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发表时间：
2009-07
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通讯作者：
D. Sisci;M. L. Panno;M. Salerno;M. Maggiolini;V. Pezzi;E. G. Morrone;L. Mauro;S. Aquila;S. Marsico;M. Lanzino;S. Andò
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作者：
D. Sisci;M. L. Panno;M. Salerno;M. Maggiolini;V. Pezzi;E. G. Morrone;L. Mauro;S. Aquila;S. Marsico;M. Lanzino;S. Andò
三碘甲腺原氨酸（T3）的管理，导致支持细胞在青春期前大鼠的早熟分化的影响已被证明。支持细胞的功能成熟与雄激素代谢的变化有关。我们最近发现，T3通过抑制芳香化酶活性影响睾丸支持细胞的雄激素代谢，并显著降低青春期甲状腺功能减退大鼠的雌激素受体含量。为了更好地了解T3在调节类固醇对支持细胞作用中的作用，我们进行了一项时间过程研究，评估T3和睾酮（T）对分离自2周龄Wistar大鼠的支持细胞中雄激素（AR）和雌激素（ER）受体含量的体外影响。在时间过程研究期间，AR和ER基础水平没有变化，这表明暴露于培养基本身并不影响两种受体类型。在与T3或T孵育24小时后，观察到细胞核和细胞质中的ER减少。同时给予两种激素可增强这种降低。AR在两个细胞区室中显示不同的时间模式，并且在T3或T诱导的胞质溶胶中表现出较早的上升。在36小时，AR显着增强，在两个隔室响应于T或T3暴露，而联合激素治疗引起的累加增加相比，单一的治疗组。由于AR和ER表现出相反的行为模式，在暴露于激素处理24小时后，总AR和ER含量之间的比率增加。为了评估所进行的处理是否诱导支持细胞的功能性成熟，测量培养基中的转铁蛋白水平。该蛋白的增加与ARs含量和ARs/ERs比值的增加相一致。这项研究表明，甲状腺激素诱导的（AR）/（ER）的比例进行性增加，在分化的支持细胞，使他们成为一个普遍的雄激素依赖沿着他们的功能成熟。
The effect of Tri-iodothyronine (T3) administration leading to the precocius differentiation of Sertoli cell in prepuberal rats has been previously shown. The functional maturation of Sertoli cells is associated with changes in androgen metabolism. We have recently demonstrated that T3 influences androgen metabolism in Sertoli cells by inhibiting aromatase activity and reduces drastically the ER contents in peripubertal hypothyroid rats. To better understand the role of T3 in modulating steroid action on Sertoli cells, we performed a time course study evaluating the in vitro effects of T3 and testosterone (T) on androgen (ARs) and estrogen (ERs) receptor content in Sertoli cells isolated from two weeks old Wistar rats. ARs and ERs basal levels did not change during the time course study indicating that the exposure to culture medium per se did not affect either receptor type. After 24 hrs of incubation with either T3 or T, a decrease of ERs in both nucleus and cytosol was observed. Such a decrease was augmented by the simultaneous administration of both hormones. ARs displayed a different temporal pattern in the two cellular compartments and exhibited an earlier rise in the cytosol induced by either T3 or T. At 36 hrs, ARs were significantly enhanced in both compartments in response to either T or T3 exposure while combined hormonal treatment caused an additive increase compared with the single treatment group. As a consequence of the opposite behaviour pattern displayed by ARs and ERs, the ratio between total ARs and ERs contents was increased after 24 hrs of exposure to hormonal treatment. To evaluate if treatments performed induced a functional maturation of Sertoli cells, transferrin levels in culture medium were measured. The increase of this protein paralleled that of ARs content as well as that of ARs/ERs ratio. This study demonstrates that thyroid hormone induces a progressive increase of (AR)/(ER) ratio in the differentiating Sertoli cells bringing them to a prevalent androgen dependency along their functional maturation.