3-Hydroxyphthalic Anhydride- Modified Rabbit Anti-PAP IgG as a Potential Bifunctional HIV-1 Entry Inhibitor.
3-Hydroxyphthalic Anhydride- Modified Rabbit Anti-PAP IgG as a Potential Bifunctional HIV-1 Entry Inhibitor.
复制标题
3-羟基邻苯二甲酸酐修饰的兔抗 PAP IgG 作为潜在的双功能 HIV-1 进入抑制剂
DOI：
10.3389/fmicb.2018.01330
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发表时间：
2018
影响因子：
5.2
通讯作者：
Li L
中科院分区：
文献类型：
--
作者：
Zhang X;Chen J;Yu F;Wang C;Ren R;Wang Q;Tan S;Jiang S;Liu S;Li L
几项研究报告说，由预周期酸性磷酸酶的天然肽片段形成的人类精液（PAP248-286），被称为病毒感染的精液增强子（SEVI），可以极大地增强人类免疫缺陷型药物（HEIV型），这可能会受到新的影响。为了预防性传播的艾滋病毒。理论上，特殊的抗PAP或抗SEVI抗体可以通过阻止HIV和SEVI原纤维的结合来减少病毒感染的增强，3-羟基苯甲酸半甲状腺肿瘤改性的抗PAP248-286抗体，使用HP-PPECTION和高度群体的群体， ，细胞 - 细胞融合和单周期HIV-1感染测定法，我们证明了HP-API是HIV-1进入/融合抑制剂。根据这些发现，HP-API可以被认为是具有高潜力的双功能HIV-1进入/融合抑制剂。
Several studies have reported that amyloid fibrils in human semen formed from a naturally occurring peptide fragment of prostatic acidic phosphatase (PAP248-286), known as semen-derived enhancer of viral infection (SEVI), could dramatically enhance human immunodeficiency virus type 1 (HIV-1) infection. Accordingly, SEVI might serve as a novel target for new antiviral drugs or microbicide candidates for the prevention of sexually transmitted HIV. Theoretically, a special anti-PAP or anti-SEVI antibody could reduce the enhancement of viral infection by blocking the binding of HIV and SEVI fibrils. Here, 3-hydroxyphthalic anhydride modified anti-PAP248-286 antibody, named HP-API, exhibited broad-spectrum and highly effective anti-HIV-1 activities on different subtypes and tropism. By using time-of-addition, cell–cell fusion and a single-cycle HIV-1 infection assays, we demonstrated that HP-API is an HIV-1 entry/fusion inhibitor. Mechanism studies suggest that HP-API inhibited HIV-1 entry/fusion by targeting both HIV-1 gp120 envelop and CD4 receptor on the host cell specifically. It is noteworthy that HP-API abrogated the formation of SEVI fibrils and partially interfered with SEVI-mediated enhancement of HIV-1 infection. Based on these findings, HP-API could be considered a bifunctional HIV-1 entry/fusion inhibitor with high potential.
影响因子：
3.7
作者：
Schwalbe B;Schreiber M
通讯作者：
Schreiber M