Acquisition of fear conditioning in rats requires the synthesis of mRNA in the amygdala
Acquisition of fear conditioning in rats requires the synthesis of mRNA in the amygdala
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DOI：
10.1037/0735-7044.113.2.276
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发表时间：
1999-04-01
影响因子：
1.9
通讯作者：
Helmstetter, FJ
中科院分区：
文献类型：
--
作者：
Bailey, DJ;Kim, JJ;Helmstetter, FJ
在这项研究中，研究了条件性恐惧获得过程中杏仁核中 mRNA 合成的作用。在杏仁核基底外侧区域放置插管的大鼠在一个独特的观察室中接受一系列噪声电击配对的训练。一半的大鼠接受了 mRNA 合成抑制剂放线菌素-D (act-D) 的预处理。通过防御性冻结来评估对训练环境和新环境中听觉刺激的反应。 act-D 预处理显着减弱了对两种刺激的恐惧反应。接受act-D注射的动物在初始训练中对条件刺激-非条件刺激配对表现出正常反应，并且在注射后7天重新训练时表现出正常学习。这些结果表明，杏仁核中新 mRNA 的转录和随后的蛋白质合成可能对于这种形式的联想学习过程中的神经可塑性至关重要。
In this study, the role of mRNA synthesis in the amygdala was studied during the acquisition of conditional fear. Rats with cannulas placed in the basolateral region of the amygdala were trained with a series of noise-shock pairings in a distinctive observation chamber. One half of the rats were pretreated with the mRNA synthesis inhibitor actinomycin-D (act-D). Responding to the training context and the auditory stimulus in a novel context measured by defensive freezing was assessed. Pretreatment with act-D significantly attenuated fear responses to both stimuli. Animals receiving act-D injections exhibited normal reactions to the conditioned stimulus-unconditioned stimulus pairings in the initial training session and displayed normal learning when retrained 7 days after injections. These results indicate that the transcription of new mRNA and subsequent protein synthesis in the amygdala may be essential for neural plasticity during this form of associative learning.