The highly related DEAD box RNA helicases p68 and p72 exist as heterodimers in cells
The highly related DEAD box RNA helicases p68 and p72 exist as heterodimers in cells
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DOI：
10.1093/nar/gkg236
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发表时间：
2003-03-01
影响因子：
14.9
通讯作者：
Fuller-Pace, FV
中科院分区：
文献类型：
--
作者：
Ogilvie, VC;Wilson, BJ;Fuller-Pace, FV
RNA 解旋酶 p68 和 p72 是 DEAD box 蛋白家族的高度相关成员，在保守核心之间具有 90% 的同一性，并且已被证明参与转录和 mRNA 加工。我们之前表明这些蛋白质共定位于间期细胞的细胞核中。在这项研究中，我们证明 p68 和 p72 可以在酵母双杂交系统中相互作用并自缔合。免疫共沉淀实验证实 p68 和 p72 可以在细胞中相互作用，并表明这些蛋白质优先以异二聚体形式存在。此外，我们还发现 p68 可以与 NFAR-2 相互作用，NFAR-2 是一种也被认为在 mRNA 加工中发挥作用的蛋白质。此外，凝胶过滤分析表明，p68 和 p72 可以存在于细胞中的多种复合物中（大小范围从大约 150 kDa 到大约 400 kDa），其中 p68 分子的子集存在于非常大的复合物中（>2 MDa）。存在于可能含有 p68 和/或 p72 的不同复合物中的潜力，以及一系列其他因素，将为这些蛋白质提供与不同 RNA 底物相互作用的潜力，并且与最近的报告一致，暗示 p68/p72 具有广泛的功能。
The RNA helicases p68 and p72 are highly related members of the DEAD box family of proteins, sharing 90% identity across the conserved core, and have been shown to be involved in both transcription and mRNA processing. We previously showed that these proteins co-localise in the nucleus of interphase cells. In this study we show that p68 and p72 can interact with each other and self-associate in the yeast two-hybrid system. Co-immunoprecipitation experiments confirmed that p68 and p72 can interact in the cell and indicated that these proteins preferentially exist as hetero-dimers. In addition, we show that p68 can interact with NFAR-2, a protein that is also thought to function in mRNA processing. Moreover, gel filtration analysis suggests that p68 and p72 can exist in a variety of complexes in the cell (ranging from similar to150 to similar to400 kDa in size), with a subset of p68 molecules being in very large complexes (>2 MDa). The potential to exist in different complexes that may contain p68 and/or p72, together with a range of other factors, would provide the potential for these proteins to interact with different RNA substrates and would be consistent with recent reports implying a wide range of functions for p68/p72.