HYALURONIC ACID IN SYNOVIAL FLUID .I. MOLECULAR PARAMETERS OF HYALURONIC ACID IN NORMAL AND ARTHRITIC HUMAN FLUIDS
HYALURONIC ACID IN SYNOVIAL FLUID .I. MOLECULAR PARAMETERS OF HYALURONIC ACID IN NORMAL AND ARTHRITIC HUMAN FLUIDS
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DOI：
10.1002/art.1780100407
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发表时间：
1967-01-01
影响因子：
--
通讯作者：
ROSEMAN, S
中科院分区：
文献类型：
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作者：
BALAZS, EA;WATSON, D;ROSEMAN, S
采集268名21~45岁正常成年男性志愿者膝关节滑液，用生理盐水冲洗关节腔。测定滑液和关节冲洗液中透明质酸、蛋白质和唾液酸的浓度及极限粘数。采用电沉积法分离透明质酸，通过沉降法、扩散法、粘度法、双折射流法和光散射法计算了透明质酸的分子参数。对3例关节炎关节滑液标本(供体85例，年龄17~72岁)进行分析，并用于制备纯透明质酸。结果表明，透明质酸的极限粘数、平均相对分子质量和分子直径均明显小于由正常关节液制备的透明质酸。测定了4例类风湿关节炎患者关节液中透明质酸、蛋白质和唾液酸的含量及透明质酸的极限粘数。在类风湿性关节炎中，关节和相连腔的总透明质酸和蛋白质含量增加。同时，蛋白质中唾液酸和己胺的含量也有所增加。类风湿滑液及其制备的透明质酸表现出非牛顿流动行为，但程度明显低于正常滑液。正常和类风湿关节炎滑液的流变特性差异可能源于透明质酸的浓度、分子参数和分子相互作用的不同。
Synovial fluid from the knee joints of 268 normal adult male volunteers (age 21-45 years) was collected, and the joint cavities were washed with physiological saline solution. The hyaluronic acid, protein and sialic acid concentrations and the limiting viscosity numbers were determined in both synovial fluid and joint washings. The hyaluronic acid was separated by electrodeposition, and its molecular parameters calculated from sedimentation, diffusion, viscosity, double refraction of flow, and light-scattering measurements. Three pooled samples of arthritic synovial fluids (85 donors, ages 17-72) were analyzed and used for the preparation of pure hyaluronic acid. The limiting viscosity number, average molecular weight and molecular diameter were found to be significantly smaller than those of the hyaluronic acid prepared from normal joint fluid. The hyaluronic acid, protein and sialic acid content, and limiting viscosity number of hyaluronic acid were determined in 7 individual samples of joint fluids obtained from four patients with rheumatoid arthritis. In rheumatoid arthritis the total hyaluronic acid and protein content of the joint and connected cavities is increased. At the same time, the sialic acid and hexos-amine content of the proteins is also elevated. Rheumatoid synovial fluid and the hyaluronic acid prepared from it exhibit non-Newtonian flow behavior but to a significantly lesser degree than normal synovial fluid. The differences between the rheological characteristics of the normal and rheumatoid arthritis synovial fluid may originate from the differences in the concentration, molecular parameters and molecular interaction of hyaluronic acid.