EFFECTS OF DUCT LIGATION ON FERTILIZING ABILITY OF SPERMATOZOA FROM DIFFERENT REGIONS OF RABBIT EPIDIDYMIS
EFFECTS OF DUCT LIGATION ON FERTILIZING ABILITY OF SPERMATOZOA FROM DIFFERENT REGIONS OF RABBIT EPIDIDYMIS
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DOI：
10.1002/jez.1401660210
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发表时间：
1967-01-01
影响因子：
--
通讯作者：
BEDFORD, JM
中科院分区：
文献类型：
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作者：
BEDFORD, JM
正常家兔，可育精子首先出现在附睾下半部分。如果精子在低睾丸区域的通道受阻，受精和启动正常卵子发育的能力最终会在附睾管屈曲末端的任何部分产生。从附睾屈曲头近端的起始部分释放的精子没有受精，尽管许多精子在低结扎后12 1/2至15天表现出明显正常的进行性运动。由于这些不育的、有活力的精子在含有血清的培养基中没有表现出成熟兔子精子正常的头-头凝集反应，因此，活力和受精能力之间的对立可能至少部分是由于精子质膜持续处于未成熟状态。许多表现出正常持续运动能力的头精子在颈部区域保留了细胞质液滴，而在其他表现出类似运动能力的精子中，液滴在颈部区域，而在其他表现出类似运动能力的精子中，液滴沿着中部移动或丢失。因此，在成熟精子运动能力的发展中，液滴的运动和丢失似乎不太可能是重要的。
In normal rabbit, fertile spermatozoa first appear in the lower half of the corpus epididymidis. If sperm passage is impeded in the low corpus region, the capacity to fertilize and initiate normal ovum development can ultimately arise in sperm withheld in any part of the ductus epididymidis, distal to the caput flexure. Sperm released from the initial part of the epididymis proximal to the caput flexure did not fertilize, even though many showed apparently normal progressive motility 12 1/2 to 15 days after low corporeal ligation. Since these infertile, motile sperm did not show the normal head-head agglutination reaction of mature rabbit sperm in a medium containing serum, it appears that the dichotomy between motility and fertilizing ability may have been at least partly due to the persistence in an immature state of the sperm plasma membrane. Many caput sperm which displayed normal sustained motility had retained the cytoplasmic droplet in the neck region, whereas, in others showing comparable motility, the droplet in the neck region, whereas, in others showing comparable motility, the droplet had moved along the midpiece or had been lost. It, therefore, seems unlikely that movement and loss of the droplet can be of importance in development of the capacity for motility in maturing spermatozoa.