A compact functional quantum Dot-DNA conjugate: preparation, hybridization, and specific label-free DNA detection.
A compact functional quantum Dot-DNA conjugate: preparation, hybridization, and specific label-free DNA detection.
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DOI：
10.1021/la703583u
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发表时间：
2008-01
期刊：
影响因子：
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通讯作者：
Dejian Zhou;L. Ying;X. Hong;E. Hall;C. Abell;D. Klenerman
中科院分区：
文献类型：
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作者：
Dejian Zhou;L. Ying;X. Hong;E. Hall;C. Abell;D. Klenerman
在这封信中，我们报告了一个紧凑的，功能性的量子点（QD）-DNA结合物的制备，其中捕获的目标DNA是直接和共价耦合到QD表面。这使得能够将QD供体和染料受体之间的分离距离控制在Förster半径的范围内。此外，三（乙二醇）接头被引入到量子点表面涂层，以有效地消除强，非特异性吸附的DNA上的量子点表面。结果，该QD-DNA缀合物与其互补DNA特异性杂交，其杂交速率常数与溶液中游离DNA的杂交速率常数相当。我们表明，该系统能够通过“信号开启”荧光共振能量转移（FRET）响应，在低DNA探针/QD拷贝数下特异性检测纳摩尔未标记的互补DNA。
In this letter, we report the preparation of a compact, functional quantum dot (QD)-DNA conjugate, where the capturing target DNA is directly and covalently coupled to the QD surface. This enables control of the separation distance between the QD donor and dye acceptor to within the range of the Förster radius. Moreover, a tri(ethylene glycol) linker is introduced to the QD surface coating to effectively eliminate the strong, nonspecific adsorption of DNA on the QD surface. As a result, this QD-DNA conjugate hybridizes specifically to its complementary DNA with a hybridization rate constant comparable to that of free DNAs in solution. We show this system is capable of specific detection of nanomolar unlabeled complimentary DNA at low DNA probe/QD copy numbers via a "signal-on" fluorescence resonance energy transfer (FRET) response.