Analysis of DNA-vaccinated fish reveals viral antigen in muscle, kidney and thymus, and transient histopathologic changes
Analysis of DNA-vaccinated fish reveals viral antigen in muscle, kidney and thymus, and transient histopathologic changes
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DOI：
10.1007/s10126-004-5129-z
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发表时间：
2005-09-01
影响因子：
3
通讯作者：
Kurath, G
中科院分区：
文献类型：
--
作者：
Garver, KA;Conway, CM;Kurath, G
针对鱼类弹状病毒，传染性造血细胞坏死病毒（IHNV）的高效DNA疫苗用于系统研究，以分析疫苗组织分布、持久性、表达模式和组织病理学效应。在肌肉注射后立即在所有分析的组织（包括血液）中检测到含有病毒糖蛋白基因的疫苗质粒pIHNw-G，但在随后的时间点主要在肌肉组织中发现，并持续90天。在肌肉、肾脏和胸腺组织中检测到糖蛋白表达，其水平在14天时达到峰值，到28天时检测不到。组织学检查显示，在标准有效剂量0.1 μ g DNA/鱼时，没有疫苗特异性病理变化，但在50 μ g的高剂量下，炎症反应明显增加。与针头注射相关的短暂损伤局限于肌肉组织，但到接种后90天，在任何组织中均未检测到损伤，表明疫苗安全且耐受性良好。
A highly efficacious DNA vaccine against a fish rhabdovirus, infectious hematopoietic necrosis virus (IHNV), was used in a systematic study to analyze vaccine tissue distribution, persistence, expression patterns, and histopathologic effects. Vaccine plasmid pIHNw-G, containing the gene for the viral glycoprotein, was detected immediately after intramuscular injection in all tissues analyzed, including blood, but at later time points was found primarily in muscle tissue, where it persisted to 90 days. Glycoprotein expression was detected in muscle, kidney, and thymus tissues, with levels peaking at 14 days and becoming undetectable by 28 days. Histologic examination revealed no vaccine-specific pathologic changes at the standard effective dose of 0.1 mu g DNA per fish, but at a high dose of 50 mu g an increased inflammatory response was evident. Transient damage associated with needle injection was localized in muscle tissue, but by 90 days after vaccination no damage was detected in any tissue, indicating the vaccine to be safe and well tolerated.