Non-specific immune factors differences in coelomic fluid from polian vesicle and coelom of Apostichopus japonicus, and their early response after evisceration
Non-specific immune factors differences in coelomic fluid from polian vesicle and coelom of Apostichopus japonicus, and their early response after evisceration
复制标题
仿刺参体腔液与刺参体腔液中非特异性免疫因子的差异及其开膛后的早期反应
DOI：
10.1016/j.fsi.2019.12.094
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发表时间：
2020-03-01
影响因子：
4.7
通讯作者：
Li, Qiang
中科院分区：
文献类型：
--
作者：
Ren, Yuan;Zhang, Jialin;Li, Qiang
刺参体腔液中含有大量的体腔细胞、酶、营养物质和多种分子，是刺参生存所必需的。体腔囊泡和泡囊是动物体腔液的主要贮存组织，但这两种组织的体腔液是否存在免疫学差异尚不清楚。本研究首先分别从体腔和泡囊中提取体腔液，比较其非特异性免疫因子。结果表明，泡囊内ACP和AKP活性显著高于体腔内，而SOD和CAT活性则相反;泡囊内AjC 3 -2、AjMKK 3/6、AjTLR 3和AjToll等免疫相关基因的表达水平显著低于体腔内。此外，进一步监测了去净内脏后非特异性免疫因子的早期变化。在去内脏后7天内，体腔液中ACP、AKP、SOD和CAT等免疫酶活性先下降后逐渐恢复。在囊泡中，ACP和AKP活性变化趋势与体腔相似，而SOD和CAT活性在去内脏后2 ~ 12 h呈现短暂的上升趋势。此外，体腔中免疫相关基因的表达谱在内脏取出后3天达到峰值，而在泡囊中的表达水平在7天达到峰值。上述结果提示，体腔液和泡囊的免疫活性不同，可能发挥各自的免疫功能。内脏摘除后体腔内的反应速度可能快于泡囊内的反应速度。本研究结果为进一步了解A. japonicas.
Coelomic fluid contains a population of coelomocytes, enzymes, nutrients and kinds of molecules that could be essential for Apostichopus japonicas live. The coelom and polian vesicle are the main tissues that hold the most coelomic fluid in the animal, but whether there exists any immunological difference of the coelomic fluid from the two tissues remains unknown. In this study, we first extracted the coelomic fluid both from the coelom and polian vesicle, and compared their non-specific immune factors. It was found that the ACP and AKP activities in the polian vesicle were significantly higher than those in the coelom, but it was contrary for the SOD and CAT. Meanwhile, the expression levels of several immune-related genes including AjC3-2, AjMKK3/6, AjTLR3 and AjToll in the polian vesicle were significantly lower than those in the coelom. Besides, the early changes of nonspecific immune factors were further monitored after eviscerated. During 7 days post evisceration, the immunoenzymes activities of ACP, AKP, SOD and CAT were decreased first and then recovered gradually in the coelomic fluid from the coelom. In the polian vesicle, the ACP and AKP activities showed a similar trend with the coelom, while the SOD and CAT activities showed a transitory increase during 2 h post evisceration (hpe) to 12 hpe. Moreover, the expression profiles of the immune-related genes in the coelom reached the peak at 3 days post evisceration (dpe), while their expression levels in the polian vesicle reached the peak at 7 dpe. All the results suggested that the immunocompetence of coelomic fluid differed in the coelom and polian vesicle, and thus may exert their respective immunological functions. It was likely that the respond speed in the coelom would be faster than that in the polian vesicle after evisceration. Our data will provide a basis for better understanding of the immune defense mechanism of A. japonicas.