Intraspecific variation in survival and mitochondrial oxidative phosphorylation in wild-caught Drosophila simulans.
Intraspecific variation in survival and mitochondrial oxidative phosphorylation in wild-caught Drosophila simulans.
复制标题
野生捕获的模拟果蝇存活率和线粒体氧化磷酸化的种内变异。
DOI：
10.1111/j.1474-9726.2006.00211.x
复制
发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Ballard,JWilliamO
中科院分区：
文献类型：
--
作者：
Melvin,RichardG;Ballard,JWilliamO
即使在同一物种的个体之间，生物体的寿命也有很大差异。在衰老的氧自由基理论的框架下，预测物种内个体寿命的变化将与活性氧对细胞组分的氧化损伤积累的变化相关。在这项研究中，我们测试的假设，即三个野生caughtDrosophila simulansfly线（HW 09，NC 48和MD 106）的生存变化与线粒体生物能量学的三个关键方面相关。中位生存期顺序为HW 09> MD 106> NC 48。年轻的HW 09果蝇（11-18天）具有（i）最高的ADP：O（线粒体消耗的氧气量时，提供了一定量的ADP）时，代谢电子传递链复合物I和复合物III的底物;（ii）线粒体过氧化氢生产率最低的复合物III;和（iii）最高的复合物IV的细胞色素氧化酶活性。在11-25日龄范围内，所有品系的过氧化氢产生速率增加，细胞色素氧化酶活性降低。这是第一项将生物体生存的自然变化与线粒体生物能量学的自然变化相关联的研究。
Lifespans of organisms vary greatly even among individuals of the same species. Under the framework of the free oxygen radical theory of aging, it is predicted that variation in individual lifespan within a species will correlate with variation in the accumulation of oxidative damage to cell components from reactive oxygen species. In this study we test the hypothesis that variation in survival of three wild‐caughtDrosophila simulansfly lines (HW09, NC48 and MD106) correlates with three key aspects of mitochondrial bioenergetics. The rank order of median survival was HW09 > MD106 > NC48. Young HW09 flies (11–18 days) had (i) highest ADP:O (quantity of oxygen consumed by mitochondria when provided with a quantity of ADP) when metabolizing both electron transport chain complex I and complex III substrates; (ii) lowest rate of mitochondrial hydrogen peroxide production from complex III; and (iii) highest cytochromecoxidase activity from complex IV. Rate of hydrogen peroxide production increased and cytochromecoxidase activity decreased in all lines in the age range 11–25 days. This is the first study to correlate natural variation in organism survival with natural variation in mitochondrial bioenergetics.