Next-Generation Cancer Biomarkers: Extracellular Vesicle DNA as a Circulating Surrogate of Tumor DNA.
Next-Generation Cancer Biomarkers: Extracellular Vesicle DNA as a Circulating Surrogate of Tumor DNA.
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DOI：
10.3389/fcell.2020.622048
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发表时间：
2020
影响因子：
5.5
通讯作者：
Buscail E
中科院分区：
文献类型：
--
作者：
Amintas S;Vendrely V;Dupin C;Buscail L;Laurent C;Bournet B;Merlio JP;Bedel A;Moreau-Gaudry F;Boutin J;Dabernat S;Buscail E
细胞外囊泡（EV）由健康组织和肿瘤细胞产生，并在包括血液在内的各种体液中释放。它们受到双层磷脂膜的限制，并携带丰富的生物分子。它们的释放净化细胞的废物或作为功能性的局部和远距离细胞间通讯和分子交换颗粒。这种丰富和异质的内容在癌症病理生理学中受到了高度关注，因为EV支持癌症控制和进展。由于其特定的活性货物，它们被评估为液体活检生物标志物的载体。与可溶性循环生物标志物相比，它们的复杂性可能提供关于肿瘤和转移状态的丰富信息。由于在致癌过程中通常观察到的获得性基因组变化，EV中的双链DNA（dsDNA）可能是肿瘤存在和复杂性的最新最有前途的生物标志物。本文就EV-DNA在囊泡中的包合、EV-DNA的检测和定量技术以及EV-DNA作为临床生物标志物的应用作一综述。
Extracellular vesicles (EVs) are produced by healthy tissues and tumor cells and are released in various bodily fluids, including blood. They are limited by bilayer phospholipidic membranes, and they carry a rich content in biomolecules. Their release cleanses the cells of their waste or serves as functional local and distant cell–cell communication and molecular exchange particles. This rich and heterogeneous content has been given intense attention in cancer physiopathology because EVs support cancer control and progression. Because of their specific active cargo, they are being evaluated as carriers of liquid biopsy biomarkers. Compared to soluble circulating biomarkers, their complexity might provide rich information on tumor and metastases status. Thanks to the acquired genomic changes commonly observed in oncogenic processes, double-stranded DNA (dsDNA) in EVs might be the latest most promising biomarker of tumor presence and complexity. This review will focus on the recent knowledge on the DNA inclusion in vesicles, the technical aspects of EV-DNA detection and quantification, and the use of EV-DNA as a clinical biomarker.
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DOI：
10.1093/annonc/mdx765
发表时间：
2018-03-01
期刊：
Annals of oncology : official journal of the European Society for Medical Oncology
影响因子：
--
作者：
Krug AK;Enderle D;Karlovich C;Priewasser T;Bentink S;Spiel A;Brinkmann K;Emenegger J;Grimm DG;Castellanos-Rizaldos E;Goldman JW;Sequist LV;Soria JC;Camidge DR;Gadgeel SM;Wakelee HA;Raponi M;Noerholm M;Skog J
通讯作者：
Skog J
DOI：
10.1016/j.nano.2017.12.009
发表时间：
2018-04-01
影响因子：
5.4
作者：
Klump, Jennifer;Phillipp, Ulrike;Nazarenko, Irina
通讯作者：
Nazarenko, Irina
影响因子：
4.6
作者：
Brennan, K.;Martin, K.;Mc Gee, M. M.
通讯作者：
Mc Gee, M. M.
影响因子：
5
作者：
Buscail, Etienne;Chauvet, Alexandre;Dabernat, Sandrine
通讯作者：
Dabernat, Sandrine
影响因子：
4
作者：
Hur, Jae Young;Lee, Jong Sik;Lee, Kye Young
通讯作者：
Lee, Kye Young