Comparative Study of Biphasic Calcium Phosphates With Different HA/TCP Ratios in Mandibular Bone Defects. A Long-Term Histomorphometric Study in Minipigs
Comparative Study of Biphasic Calcium Phosphates With Different HA/TCP Ratios in Mandibular Bone Defects. A Long-Term Histomorphometric Study in Minipigs
复制标题
DOI：
10.1002/jbm.b.31271
复制
发表时间：
2009-07-01
影响因子：
3.4
通讯作者：
Buser, Daniel
中科院分区：
文献类型：
--
作者：
Jensen, Simon Storgard;Bornstein, Michael M.;Buser, Daniel
将羟基磷灰石（HA）/磷酸三钙（TCP）比例为20/80、60/40和80/20的三种双相磷酸钙（BCP）骨替代材料与凝块、颗粒状自体骨和脱蛋白牛骨矿物质（DBBM）在膜保护骨缺损中进行比较。在24只小型猪的下颌骨中制备缺损，将其分为4组，每组6只，愈合时间分别为4，13，26和52周。组织学和组织形态学评价的重点是骨形成的数量和模式、填料降解以及骨和填料之间的界面差异。膨胀聚四氟乙烯屏障膜塌陷到凝块缺陷中，强调了填充材料维持增大体积的必要性。定量分析显示，BCP 20/80的骨形成和填充材料降解与自体移植物相似，而BCP 60/40和BCP 80/20相当于DBBM。在三种BCP中，骨形成量和填充材料的降解似乎与HA/TCP比率成反比。在所有时间点，自体移植物的填充物表面被骨覆盖的分数最高，并且在早期愈合阶段DBBM高于BCP 80/20和60/40。在BCP和DBBM表面发现TRAP阳性多核细胞，但未显示吸收陷窝的典型体征。(C)2008 Wiley Periodicals，Inc.生物医学材料研究杂志B部分：应用生物材料90 B：171-181，2009年
Three biphasic calcium phosphate (BCP) bone substitute materials with hydroxyapatite (HA)/tricalcium phosphate (TCP) ratios of 20/80, 60/40, and 80/20 were compared to coagulum, particulated autogenous bone, and deproteinized bovine bone mineral (DBBM) in membrane-protected bone defects. The defects were prepared in the mandibles of 24 minipigs that were divided into four groups of six with healing times of 4, 13, 26, and 52 weeks, respectively. The histologic and histomorphometric evaluation focused on differences in amount and pattern of bone formation, filler degradation, and the interface between bone and filler. Collapse of the expanded polytetrafluoroethylene barrier membrane into the coagulum defects underlined the necessity of a filler material to maintain the augmented volume. Quantitatively, BCP 20/80 showed bone formation and degradation of the filler material similar to autografts, whereas BCP 60/40 and BCP 80/20 rather equaled DBBM. Among the three BCP's, the amount of bone formation and degradation of filler material seemed to be inversely proportional to the HA/TCP ratio. The fraction of filler surface covered with bone was highest for autografts at all time points and was higher for DBBM than BCP 80/20 and 60/40 at the early healing phase. TRAP-positive multinucleated cells were identified on BCP and DBBM surfaces without showing typical signs of resorption lacunae. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 90B: 171-181, 2009