Cloning, characterization, and nucleotide sequence analysis of the argH gene from Campylobacter jejuni TGH9011 encoding argininosuccinate lyase
Cloning, characterization, and nucleotide sequence analysis of the argH gene from Campylobacter jejuni TGH9011 encoding argininosuccinate lyase
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编码精氨琥珀酸裂合酶的空肠弯曲杆菌 TGH9011 argH 基因的克隆、表征和核苷酸序列分析
DOI：
10.1128/jb.176.7.1865-1871.1994
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发表时间：
1994
影响因子：
3.2
通讯作者：
V. Chan
中科院分区：
文献类型：
--
作者：
E. Hani;V. Chan
空肠弯曲杆菌TGH9011精氨酸琥珀酸裂解酶（argH）的完整结构基因通过与大肠杆菌argH营养缺陷突变体的互补克隆到大肠杆菌中。该基因已在4.1 kb DNA片段上进行亚克隆测序，并通过缺失突变体的互补活性进行定位。测定了空肠梭菌argH基因的完整DNA序列。通过引物延伸分析确定argH mRNA的转录起点位于上游基因磷酸烯醇丙酮酸羧激酶基因（phosphoenolpyruvate carboxykinase gene, ppc）的编码序列内。精氨酸琥珀酸裂解酶和磷酸烯醇丙酮酸羧激酶阅读框有一个碱基重叠，这是空肠梭菌染色体基因中这种现象的第二个例子。该酶的亚基分子量推断为51831。含有argH基因的重组质粒在大肠杆菌大细胞中产生56-kDa蛋白和43-kDa蛋白。在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳中观察到的较小的蛋白质可能是由于交替翻译起始产生了分子量为42 kDa的多肽。空肠C. argH基因与酵母和人精氨酸琥珀酸裂解酶基因具有核苷酸同源性，且在相应蛋白之间存在明显的保守氨基酸结构域。
The complete structural gene for argininosuccinate lyase (argH) from Campylobacter jejuni TGH9011 has been cloned into Escherichia coli by complementation of an E. coli argH auxotrophic mutant. The gene has been subcloned for sequencing on a 4.1-kb DNA segment and localized by the complementing activity of deletion mutants. The complete DNA sequence of the C. jejuni argH gene was determined. The transcription start point for argH mRNA was determined by primer extension analysis and found to be within the coding sequence of the upstream gene, identified as the phosphoenolpyruvate carboxykinase gene (ppc). The argininosuccinate lyase and the phosphoenolpyruvate carboxykinase reading frames overlap by one base, the second example of this phenomenon in C. jejuni chromosomal genes. The enzyme has a deduced subunit molecular weight of 51,831. Recombinant plasmids containing the argH gene generate a 56-kDa protein and a 43-kDa protein in E. coli maxicells. An alternate translation initiation producing a polypeptide with a deduced molecular mass of 42 kDa may account for the smaller protein observed in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The C. jejuni argH gene shows nucleotide homology to both yeast and human argininosuccinate lyase genes, and conserved amino acid domains are evident between the corresponding proteins.
DOI：
10.1016/s0021-9258(18)45347-1
发表时间：
1987-09
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
M. Wu;A. Tzagoloff
通讯作者：
M. Wu;A. Tzagoloff
人精氨酸琥珀酸裂合酶 cDNA 的克隆和序列分析。
DOI：
10.1073/pnas.83.19.7211
发表时间：
1986
影响因子：
11.1
作者：
O'Brien,WE;McInnes,R;Kalumuck,K;Adcock,M
通讯作者：
Adcock,M
影响因子：
3.5
作者：
HENIKOFF, S
通讯作者：
HENIKOFF, S