Direct and negative regulation of the sycO-ypkA-ypoJ operon by cyclic AMP receptor protein (CRP) in Yersinia pestis.
Direct and negative regulation of the sycO-ypkA-ypoJ operon by cyclic AMP receptor protein (CRP) in Yersinia pestis.
复制标题
鼠疫耶尔森氏菌环 AMP 受体蛋白 (CRP) 对 sycO-ypkA-ypoJ 操纵子的直接和负调控
DOI：
10.1186/1471-2180-9-178
复制
发表时间：
2009-08-25
期刊：
影响因子：
4.2
通讯作者：
Yang R
中科院分区：
文献类型：
--
作者：
Zhan L;Yang L;Zhou L;Li Y;Gao H;Guo Z;Zhang L;Qin C;Zhou D;Yang R
背景
包括鼠疫耶尔森氏菌在内的致病性耶尔森氏菌共用一个III型分泌系统(T3SS)，该系统由一个分泌装置、一组易位蛋白、一个控制系统以及YpkA和YopJ等6个Yop效应蛋白组成。新近发现的环状AMP受体蛋白(CRP)是一种全球调节因子，可调节鼠疫杆菌的侧向获得性基因(plA和Pst)。C反应蛋白对T3SS成分的调节尚不清楚。
结果
在鼠疫杆菌中，syco、ypkA和yopJ基因构成了一个操纵子。CRP与Syco启动子近端区域特异性结合，抑制Syco-ypkA-yopJ操纵子的表达。Syco-ypkA-yopJ操纵子使用单个依赖于CRP的启动子，但在启动子区域检测到两个CRP结合位点(位点1和位点2)。CRP结合位点、转录起始点和核心启动子元件(-10和-35区)的测定有助于我们描绘CRP依赖的启动子的结构组织，给出Syco-ypkA-yopJ的CRP-启动子DNA相互作用图。
结论
在鼠疫杆菌中，Syco-ypkA-yopJ操纵子受到C反应蛋白的直接负调控。Syco-ypkA-yopJ启动子近似区在鼠疫杆菌、假结核杆菌和小肠结肠炎耶尔森菌中极其保守。因此，本文提供的数据可以普遍适用于上述三种致病耶尔森氏菌。
Background
Pathogenic yersiniae, including Y. pestis, share a type III secretion system (T3SS) that is composed of a secretion machinery, a set of translocation proteins, a control system, and six Yop effector proteins including YpkA and YopJ. The cyclic AMP receptor protein (CRP), a global regulator, was recently found to regulate the laterally acquired genes (pla and pst) in Y. pestis. The regulation of T3SS components by CRP is unknown.
Results
The sycO, ypkA and yopJ genes constitute a single operon in Y. pestis. CRP specifically binds to the promoter-proximate region of sycO, and represses the expression of the sycO-ypkA-yopJ operon. A single CRP-dependent promoter is employed for the sycO-ypkA-yopJ operon, but two CRP binding sites (site 1 and site 2) are detected within the promoter region. A CRP box homologue is found in site 1 other than site 2. The determination of CRP-binding sites, transcription start site and core promoter element (-10 and -35 regions) promotes us to depict the structural organization of CRP-dependent promoter, giving a map of CRP-promoter DNA interaction for sycO-ypkA-yopJ.
Conclusion
The sycO-ypkA-yopJ operon is under the direct and negative regulation of CRP in Y. pestis. The sycO-ypkA-yopJ promoter-proximate regions are extremely conserved in Y. pestis, Y. pseudotuberculosis and Y. enterocolitica. Therefore, data presented here can be generally applied to the above three pathogenic yersiniae.
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影响因子：
3.1
作者：
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通讯作者：
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DOI：
10.1073/pnas.94.1.265
发表时间：
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影响因子：
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DOI：
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DOI：
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发表时间：
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影响因子：
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作者：
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影响因子：
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