Community dynamics of duckweed-associated bacteria upon inoculation of plant growth-promoting bacteria
Community dynamics of duckweed-associated bacteria upon inoculation of plant growth-promoting bacteria
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DOI：
10.1093/femsec/fiaa101
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发表时间：
2020-07-01
影响因子：
4.2
通讯作者：
Ike, Michihiko
中科院分区：
文献类型：
--
作者：
Ishizawa, Hidehiro;Kuroda, Masashi;Ike, Michihiko
植物生长促进菌（PGPB）是一种很有前途的污水处理剂，可以提高浮萍的生物量生产效率。鉴于其在水环境中的可靠使用，本研究分析了植物定植动力学的PGPB和整个浮萍相关的细菌群落的生态后果。在3种环境细菌群落存在下，以不同的细胞密度或接种时间将PGPB菌株Aquitalea magnusonii H3接种到浮萍上。结果表明，菌株H3在9个试验中的5个试验中提高了浮萍的生长11.7-32.1%。定量PCR和扩增子测序分析表明，菌株H3成功地定殖浮萍接种后1和3 d的所有培养试验。但接种H3后，浮萍上的细菌数量显著减少，且与接种H3后的浮萍上的细菌群落相似。预测的宏基因组分析表明，细菌的趋化运动和表面附着系统相关的基因一直丰富，通过社区组装的浮萍。综上所述，菌株H3在短时间内占优势定殖浮萍，并促进浮萍生长。然而，由于天然浮萍微生物组的强大恢复力，接种PGPB没有产生持久的影响。
Plant growth-promoting bacteria (PGPB) have recently been demonstrated as a promising agent to improve wastewater treatment and biomass production efficiency of duckweed hydrocultures. With a view to their reliable use in aqueous environments, this study analysed the plant colonization dynamics of PGPB and the ecological consequences for the entire duckweed-associated bacterial community. A PGPB strain, Aquitalea magnusonii H3, was inoculated to duckweed at different cell densities or timings in the presence of three environmental bacterial communities. The results showed that strain H3 improved duckweed growth by 11.7-32.1% in five out of nine experiments. Quantitative-PCR and amplicon sequencing analyses showed that strain H3 successfully colonized duckweed after 1 and 3 d of inoculation in all cultivation tests. However, it significantly decreased in number after 7 d, and similar bacterial communities were observed on duckweed regardless of H3 inoculation. Predicted metagenome analysis suggested that genes related to bacterial chemotactic motility and surface attachment systems are consistently enriched through community assembly on duckweed. Taken together, strain H3 dominantly colonized duckweed for a short period and improved duckweed growth. However, the inoculation of the PGPB did not have a lasting impact due to the strong resilience of the natural duckweed microbiome.