High resolution mapping of Dense spike-ar (dsp.ar) to the genetic centromere of barley chromosome 7H
High resolution mapping of Dense spike-ar (dsp.ar) to the genetic centromere of barley chromosome 7H
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DOI：
10.1007/s00122-011-1712-7
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发表时间：
2012-02-01
影响因子：
5.4
通讯作者：
Stein, Nils
中科院分区：
文献类型：
--
作者：
Shahinnia, Fahimeh;Druka, Arnis;Stein, Nils
大麦的穗密度是由几个主基因控制的，如先前通过对许多形态突变体的遗传分析所证明的。一类这样的突变体影响轴节间长度，导致密集或紧凑的穗和潜在的基因被指定为密集穗（dsp）。我们在BW 265（一个BC 7 F3近等基因系）染色体3 H（21个SNP位点，35.5cM）和7 H（17个SNP位点，20.34cM）上定位了两个渐渗基因组片段。Bowman通过对BW 265及其轮回亲本中的1,536个单核苷酸多态性（SNP）标记进行基因分型，确定其可能含有密集穗突变位点dsp.ar。在此，通过高分辨率双亲作图将该基因分配到分别位于染色体7 H的遗传着丝粒的短臂和长臂上的标记SC 57808（Hv_SPL14）-CAPSK 06413之间的0.37cM间隔。通过与大麦、水稻、高粱和短柄草的共线区域比较，推断该区域包含800多个基因。由于目标基因座处遗传距离与物理距离的必然关系，因此基因的经典基于图位的分离将是复杂的。dsp.ar
Spike density in barley is under the control of several major genes, as documented previously by genetic analysis of a number of morphological mutants. One such class of mutants affects the rachis internode length leading to dense or compact spikes and the underlying genes were designated dense spike (dsp). We previously delimited two introgressed genomic segments on chromosome 3H (21 SNP loci, 35.5 cM) and 7H (17 SNP loci, 20.34 cM) in BW265, a BC7F3 nearly isogenic line (NIL) of cv. Bowman as potentially containing the dense spike mutant locus dsp.ar, by genotyping 1,536 single nucleotide polymorphism (SNP) markers in both BW265 and its recurrent parent. Here, the gene was allocated by high-resolution bi-parental mapping to a 0.37 cM interval between markers SC57808 (Hv_SPL14)-CAPSK06413 residing on the short and long arm at the genetic centromere of chromosome 7H, respectively. This region putatively contains more than 800 genes as deduced by comparison with the collinear regions of barley, rice, sorghum and Brachypodium, Classical map-based isolation of the gene dsp.ar thus will be complicated due to the infavorable relationship of genetic to physical distances at the target locus.