Detection of Eight Periodontal Microorganisms and Distribution of Porphyromonas gingivalis fimA Genotypes in Chinese Patients With Aggressive Periodontitis
Detection of Eight Periodontal Microorganisms and Distribution of Porphyromonas gingivalis fimA Genotypes in Chinese Patients With Aggressive Periodontitis
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中国侵袭性牙周炎患者牙周八种微生物检测及牙龈卟啉单胞菌fimA基因型分布
DOI：
10.1902/jop.2013.120677
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发表时间：
2014-01-01
影响因子：
4.3
通讯作者：
Wang, Xiane
中科院分区：
文献类型：
--
作者：
Feng, Xianghui;Zhang, Li;Wang, Xiane
背景：中国侵袭性牙周炎（AgP）的微生物学特征尚未确定。本研究旨在调查8种牙周微生物的患病率和牙龈卟啉单胞菌fimA基因型的分布在一个队列的AgP.Methods：唾液和混合龈下菌斑样本收集81例AgP患者（25与切牙-第一磨牙型和56与泛发型[GAgP]）和34牙周健康对照组。采用聚合酶链反应（PCR）检测8种牙周微生物，包括伴放线菌聚集杆菌、牙龈卟啉单胞菌、嗜酸坦那菌、齿垢密螺旋体、直弯杆菌、中间普雷沃菌、变黑普雷沃菌和具核梭杆菌。此外，还采用PCR方法检测了牙龈卟啉单胞菌阳性者的fimA基因型分布。Anasthia，T. denticola，齿生隐翅虫C. rectus、中间拟青霉（P. intermedia）、F. nucleatum和A. AgP患者伴放线菌数显著高于健康对照组。A.伴放线菌感染率较低（30.4%）。Logistic回归分析结果显示，年龄越小，A.伴放线菌（OR = 2.85）。结论：牙龈卟啉单胞菌、T. Anasthia，T. denticola，齿生隐翅虫C. rectus、P. intermedia和F.有核菌是我国GAgP患者的主要牙周致病菌。牙龈卟啉单胞菌fimA Ⅱ型是AgP患者中最常见的基因型。A.伴放线菌感染率相对较低。
Background: The microbiologic feature of aggressive periodontitis (AgP) in Chinese patients has not yet been determined. This study aims to investigate the prevalence of eight periodontal microorganisms and the distribution of the Porphyromonas gingivalis fimA genotype in a cohort of Chinese patients with AgP.Methods: Saliva and pooled subgingival plaque samples were collected from 81 patients with AgP (25 with incisor-first molar type and 56 with generalized type [GAgP]) and 34 periodontally healthy controls. Eight periodontal microorganisms, including Aggregatibacter actinomycetemcomitans, P. gingivalis, Tannerella forsythia, Treponema denticola, Campylobacter rectus, Prevotella intermedia, Prevotella nigrescens, and Fusobacterium nucleatum were detected in these samples by the polymerase chain reaction (PCR). In addition, the distribution of fimA genotypes was assessed in P. gingivalis-positive individuals by PCR.Results: The prevalence of P. gingivalis, T. forsythia, T. denticola, C. rectus, P. intermedia, F. nucleatum, and A. actinomycetemcomitans in patients with AgP was significantly higher than that in healthy controls. The prevalence of A. actinomycetemcomitans in patients with GAgP was relatively low (30.4%) compared with other pathogens. Results of logistic regression analysis showed that younger patients were more likely to harbor A. actinomycetemcomitans (odds ratio = 2.85). Type II was the most prevalent fimA genotype of P. gingivalis in patients with AgP.Conclusions: P. gingivalis, T. forsythia, T. denticola, C. rectus, P. intermedia, and F. nucleatum were the predominant periodontal pathogens of patients with GAgP in China. Type II of fimA was the most prevalent genotype of P. gingivalis in patients with AgP. The prevalence of A. actinomycetemcomitans in patients with GAgP was relatively low.