Contractile Characteristics of Rat Mesenteric Artery after Organ Culture
Contractile Characteristics of Rat Mesenteric Artery after Organ Culture
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DOI：
10.1292/jvms.10-0205
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发表时间：
2010-12-01
影响因子：
1.2
通讯作者：
Hara, Yukio
中科院分区：
文献类型：
--
作者：
Morita, Tomoka;Yamawaki, Hideyuki;Hara, Yukio
血管器官培养是分析药物长期疗效的有用技术。多种生长因子参与了血管重塑过程中结构和功能的变化。我们研究了器官培养的大鼠肠系膜动脉(MA)在无血清条件下的基本收缩特性和胎牛血清(FBS)的长期效应。将有[E(+)]或无[E(-)]内皮的大鼠分离的MA分为1)新鲜分离(新鲜)、2)培养3天后不加FBS(对照组)或3)加10%FBS(FBS)。在E(+)对照组，去甲肾上腺素(NA)、内皮素(ET)-1和5-羟色胺(5-HT)的最大收缩作用与新鲜心肌相似。在E(-)对照组，NA的最大收缩幅度降低，而ET-1和5-HT的最大收缩幅度与新鲜相比无明显变化。在E(+)FBS中，NA、ET-1和5-羟色胺引起的最大收缩较对照组增加。在E(-)FBS，NA和ET-1的最大收缩幅度降低，而5-羟色胺的最大收缩幅度增加。在E(+)或E(-)对照和FBS中，对NA和ET-1的敏感性从新鲜开始增加。在E(+)和E(-)对照中，对5-羟色胺的敏感性从新鲜开始下降，FBS对5-羟色胺的敏感性进一步降低。在无血清条件下，器官培养三天的大鼠MA保持了足够的收缩，从而能够分析药物的长期影响。在FBS中，对照组E(+)组NA和ET-1的最大收缩增加，E(-)组的E(-)减少，而E(+)和E(-)组5-羟色胺的最大收缩均增加。
Organ culture of blood vessels is a useful technique to analyze long-term effects of drugs. Various growth factors are responsible for structural and functional changes during vascular remodeling. We investigated I) basic contractile characteristics in organ-cultured rat mesenteric arteries (MA) in serum-free condition and 2) long-term effects of fetal bovine serum (FBS). Rat isolated MA with [E (+)] or without [E (-)] endothelium were divided into 1) freshly isolated (fresh), 2) cultured for 3 days without FBS (control) or 3) with 10% FBS (FBS). In E (+) control, maximal contraction by noradrenaline (NA), endothelin (ET)-1 and 5-hydroxytriptamine (5-HT) was similar to that in fresh. In E (-) control, maximal contraction by NA decreased whereas that by ET-1 and 5-HT didn't change from fresh. In E (+) FBS, maximal contraction by NA, ET-1, and 5-HT increased from control. In E (-) FBS, maximal contraction by NA and ET-1 decreased whereas that by 5-HT increased. In E (+) or E (-) control and FBS, sensitivity to NA and ET-1 increased from fresh. In E (+) and E (-) control, sensitivity to 5-HT decreased from fresh, and that in FBS further decreased from control. Three-day organ-cultured rat MA in serum-free condition preserved enough contraction to enable analysis for long-term effects of drug,. In FBS, maximal contractions by NA and ET-1 increased in E (+) and decreased in E (-) from control, while those by 5-HT increased in both E (+) and E (-).