Structural genomics of the Thermotoga maritima proteome implemented in a high-throughput structure determination pipeline
Structural genomics of the Thermotoga maritima proteome implemented in a high-throughput structure determination pipeline
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DOI：
10.1073/pnas.142413399
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发表时间：
2002-09-03
影响因子：
11.1
通讯作者：
Stevens, RC
中科院分区：
文献类型：
--
作者：
Lesley, SA;Kuhn, P;Stevens, RC
结构基因组学正在成为定义蛋白质结构-功能关系的主要方法。为了在基因组规模上应用这种方法，必须开发新的方法和技术来确定大量的结构。我们描述了高通量结构基因组学管道的设计和实现及其在嗜热细菌海洋热菌蛋白质组中的应用。通过使用该管道，我们成功克隆并尝试表达了预测的1,877个基因中的1,376个（73%），并确定了432个蛋白质的结晶条件，其中包括23%的T. maritima蛋白质组。TM0423甘油脱氢酶和TM0449胸苷酸合成酶补充蛋白的代表性结构作为管道最终输出的例子。
Structural genomics is emerging as a principal approach to define protein structure-function relationships. To apply this approach on a genomic scale, novel methods and technologies must be developed to determine large numbers of structures. We describe the design and implementation of a high-throughput structural genomics pipeline and its application to the proteome of the thermophilic bacterium Thermotoga maritima. By using this pipeline, we successfully cloned and attempted expression of 1,376 of the predicted 1,877 genes (73%) and have identified crystallization conditions for 432 proteins, comprising 23% of the T. maritima proteome. Representative structures from TM0423 glycerol dehydrogenase and TM0449 thymidylate synthase-complementing protein are presented as examples of final outputs from the pipeline.