Effects of nitrous acid exposure on pulmonary tissues in guinea pigs
Effects of nitrous acid exposure on pulmonary tissues in guinea pigs
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DOI：
10.3109/08958378.2010.496476
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发表时间：
2010-09-01
影响因子：
2.1
通讯作者：
Takenaka, Norimichi
中科院分区：
文献类型：
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作者：
Ohyama, Masayuki;Oka, Kenji;Takenaka, Norimichi
许多关于二氧化氮（NO(2)）对呼吸功能影响的流行病学研究可能在其测量中包括了亚硝酸盐（HONO）暴露，因为传统的NO(2)测定将HONO检测为NO(2)。一些流行病学研究和人体HONO吸入实验表明HONO与肺功能下降有关。然而，很少有动物接触HONO的实验。本研究旨在建立一个HONO产生系统，用于动物暴露实验，并评估HONO暴露与豚鼠呼吸道组织病理学改变的关系。我们将豚鼠暴露于3.6 ppm的HONO和0.3 ppm的NO(2)和1.6 ppm的一氧化氮（NO）的二次产物中4周（24小时/天）。我们进行了组织病理学分析，并在HONO暴露结束后7 h 40 min至8 h 30 min测量了特定气道阻力（sRaw）。在HONO暴露中，我们发现肺泡管中心区有肺气肿样改变，肺泡管中心区扭曲，支气管上皮细胞和平滑肌细胞扩张，支气管上皮细胞扩张。这些组织病理学结果提示，高浓度的HONO和一些NO(2)和NO可能与肺功能下降和一些呼吸道症状有关。虽然在HONO暴露组中sRaw值有升高的趋势，但在HONO暴露组中sRaw值与过滤空气组之间无统计学差异（p = 0.06，学生t检验）。
Many epidemiological studies on the effects of nitrogen dioxide (NO(2)) on respiratory function may have included nitrous acid (HONO) exposures in their measures, because conventional NO(2) assays detect HONO as NO(2). A few epidemiological studies and human HONO inhalation experiments have associated HONO with decrements in lung functions. However, there have been few HONO exposure experiments in animals. This study aims to develop a HONO generation system for the animal exposure experiments, and to assess the association of HONO exposure with histopathologic alterations in the respiratory tract of guinea pigs. We exposed the guinea pigs to 3.6 ppm HONO with secondary products of 0.3 ppm NO(2) and 1.6 ppm nitric oxide (NO) for 4 weeks (24 h/day). We conducted histopathologic analyses and measured specific airway resistance (sRaw) from 7 h 40 min to 8 h 30 min after the end of HONO exposure. We found pulmonary emphysema-like alterations in the alveolar duct centriacinar regions, distortion of the centriacinar regions of alveolar ducts with extension of the bronchial epithelial cells and smooth muscle cells, and expansion of bronchial epithelial cells, in the HONO exposure. These histopathologic results suggest that a high concentration of HONO with some NO(2) and NO may associate with decrements in lung functions and some respiratory symptoms. Although the increased tendency of the sRaw value was observed in the HONO exposure group, no statistically significant difference was found between the sRaw values from the HONO exposure group and the filtered air group (p = 0.06, student's t-test).