Immunochemical characterization and localization of endothelin ETB receptor.
Immunochemical characterization and localization of endothelin ETB receptor.
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内皮素 ETB 受体的免疫化学特征和定位。
DOI：
10.1152/ajpregu.1993.264.4.r777
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
S. Hirose
中科院分区：
文献类型：
--
作者：
H. Hagiwara;T. Nagasawa;T. Yamamoto;K. M. Lodhi;T. Ito;N. Takemura;S. Hirose
针对纯化的牛内皮素 ETB 受体产生了高度特异性的抗血清，用于确定受体亚型 ETB 的组织分布，并通过免疫组织化学方法将受体定位在肾脏、肾上腺、肺、小脑和垂体中，已知这些器官的功能受到内皮素的强烈影响。在兔子体内产生的抗血清在膜蛋白的蛋白质印迹分析中特异性识别受体条带。此外，它仅免疫沉淀 ETB，从而确定了其 ETB 特异性。通过测定抗血清可免疫沉淀的内皮素受体总数的百分比，发现 ETB 相对于 ET 受体的量因组织而异：肺 (70%)、小脑 (55%)、垂体 (50%)、肾 (25%)、肾上腺 (10%) 和睾丸 (< 2%)。这意味着，在肺中，ET 是主要形式，而在睾丸中，ETA 占主导地位，包含 > 95% 的受体。组织切片的免疫组织化学检查揭示了 ETB 内皮素受体的内皮定位。
A highly specific antiserum was raised against purified bovine endothelin ETB receptor and used to determine the tissue distribution of the receptor subtype ETB and to localize the receptor immunohistochemically in the kidney, adrenal gland, lung, cerebellum, and pituitary gland whose functions are known to be under strong influence of endothelin. The antiserum raised in a rabbit specifically recognized the receptor band on Western blot analysis of membrane proteins. Furthermore, it immunoprecipitated only ETB, establishing its ETB specificity. By determination of the percentage of the total number of the endothelin receptors that is immunoprecipitable with the antiserum, the amounts of the ETB relative to those of the ET receptors were found to vary from tissue to tissue: lung (70%), cerebellum (55%), pituitary gland (50%), kidney (25%), adrenal gland (10%), and testis (< 2%). This means that, in the lung, ET is the major form, whereas in the testis, ETA is predominant, comprising >95% of the receptors. Immunohistochemical examination of tissue sections revealed endothelium localization of the ETB endothelin receptor.