Fluorescence enhancement and reflection of the excitation light observed with a multilayered substrate
Fluorescence enhancement and reflection of the excitation light observed with a multilayered substrate
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DOI：
10.1364/ao.49.000080
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发表时间：
2010-01-01
期刊：
影响因子：
1.9
通讯作者：
Yasuda, Mitsuru
中科院分区：
文献类型：
--
作者：
Akimoto, Takuo;Yasuda, Mitsuru
使用荧光素、罗丹明 B、Cy3 和 Cy5 作为荧光团，研究了由 Ag 和 Al2O3 制成的多层基底的荧光增强。 Al2O3荧光增强的变化在Al2O3厚度0～300 nm范围内出现两个峰和一个谷，并且发现这种峰和谷周期性出现。此外，还研究了来自多层基板的激发光的反射。激发光的反射也根据 Al2O3 厚度而周期性变化，并且在荧光增强峰值的 Al2O3 厚度附近观察到最大反射。研究发现，荧光增强和激发光反射的周期性变化在很大程度上可以用简单的干涉理论导出的 lambda/4 的整数倍来解释。 (C) 2009 美国光学学会
Fluorescence enhancement from a multilayered substrate fabricated with Ag and Al2O3 was investigated using fluorescein, rhodamine B, Cy3, and Cy5 as fluorophores. The change in the fluorescence enhancement with Al2O3 had two peaks and one valley in the range from 0 to 300 nm of Al2O3 thickness, and such peaks and valley were found to appear periodically. Moreover, the reflection of the excitation light from the multilayered substrate was investigated. The reflection of the excitation light periodically changed depending on the Al2O3 thickness as well, and the maximum reflection was observed near the Al2O3 thickness of the peak fluorescence enhancement. It was found that the periodic changes of the fluorescence enhancement and the reflection of the excitation light could be explained, for the most part, with the integral multiples of the lambda/4 derived by a simple interference theory. (C) 2009 Optical Society of America