SEQUENTIAL GENE ACTIVATION BY ECDYSONE IN POLYTENE CHROMOSOMES OF DROSOPHILA-MELANOGASTER .3. CONSEQUENCES OF ECDYSONE WITHDRAWAL
SEQUENTIAL GENE ACTIVATION BY ECDYSONE IN POLYTENE CHROMOSOMES OF DROSOPHILA-MELANOGASTER .3. CONSEQUENCES OF ECDYSONE WITHDRAWAL
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DOI：
10.1016/0012-1606(76)90302-x
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发表时间：
1976-01-01
影响因子：
2.7
通讯作者：
RICHARDS, G
中科院分区：
文献类型：
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作者：
ASHBURNER, M;RICHARDS, G
蜕皮激素诱导多线果蝇唾腺染色体的一系列膨化活性变化。黑腹果蝇在蜕皮激素的持续存在下，该序列需要约12小时才能完成。将唾液腺在蜕皮激素存在下孵育不同时间，然后转移到无激素培养基中，以研究激素去除对膨化活动顺序的影响。早期蜕皮激素诱导的泡芙在去除激素后立即消退。如果再次暴露于激素，它们可以完全再诱导，但只有当暴露于激素的初始时间≤ 10分钟时。2小时;随着初始暴露时间的延长，早期喷烟逐渐难以再诱发。放线菌酮可抑制这一过程。一组的后期泡芙可以诱导过早地去除激素，其过早诱导的程度与早期泡芙的大小在蜕皮激素暴露期间。蜕皮激素抑制这些晚喷的过早诱导。
Ecdysone induces a sequence of changes in puffing activity of the polytene salivary gland chromosomes of D. melanogaster. In the continuous presence of ecdysone this sequence takes about 12 h to complete. Salivary glands were incubated in the presence of ecdysone for various times and then transferred to hormone-free medium to study the effects of hormone removal on the sequence of puffing activities. The early ecdysone-induced puffs immediately regressed on removal of hormone. They could be fully reinduced if exposed to hormone again, but only if the initial period of exposure to the hormone was .ltoreq. 2 h; with longer periods of initial exposure the early puffs became progressively refractory to reinduction. This process was inhibited by cycloheximide. A group of the late puffs could be induced prematurely by removal of the hormone; the extent of their premature induction was correlated with the size of the early puffs during the period of ecdysone exposure. Ecdysone inhibited the premature induction of these late puffs.