Sulfated modification of epimedium polysaccharide and effects of the modifiers on cellular infectivity of IBDV
Sulfated modification of epimedium polysaccharide and effects of the modifiers on cellular infectivity of IBDV
复制标题
淫羊藿多糖的硫酸化修饰及其对IBDV细胞感染性的影响
DOI：
10.1016/j.carbpol.2007.05.024
复制
发表时间：
2008-01-24
影响因子：
11.2
通讯作者：
Wang, Junmin
中科院分区：
文献类型：
--
作者：
Lu, Yu;Wang, Deyun;Wang, Junmin
通过正交试验，设计了氯磺酸-吡啶法硫酸淫羊藿多糖（EPS）的9个改性条件。获得9种磺化EPS(sEPS)，分别为sEPS(1)、sEPS(2)、sEPS(3)、sEPS(4)、sEPS(5)、sEPS(6)、sEPS(7)、sEPS(8)和sEPS(9)，并采用MTT法比较其对感染鸡胚成纤维细胞（CEF）的IBDV的抑制作用。结果表明，与未磺化的EPS相比，改性的sEPS显著增强了CEF抗IBDV感染的能力，其中以sEPS(2)和sEPS(5)的效果更好。硫酸盐基团的含量与碳水化合物含量之间存在一定的关系。最佳改性条件为反应温度为80℃，氯磺酸与吡啶的比例为1:8，反应时间为2 h。版权所有。
Nine modification conditions were designed to sulfate epimedium polysaccharide (EPS) by chlorosulfonic acid-pyridine method according to orthogonal test. and nine sulfated EPS (sEPS), sEPS(1), sEPS(2), sEPS(3), sEPS(4), sEPS(5), sEPS(6), sEPS(7), sEPS(8) and sEPS(9), were obtained and their effects on IBDV infecting chick embryo fibroblast (CEF) was compared by MTT assay. The results showed that modified sEPS had significantly enhanced CEF resisting IBDV infection in comparison of non-sulfated EPS, especially, sEPS(2) and sEPS(5) presented better effects. which there were certain relationships in the degree Of Sulfate group (DS) and carbohydrate content. The optimum modification conditions were the reaction temperature of 80 degrees C, the ratio of chlorosulfonic acid to pyridine of 1:8 and the reaction time of 2 h. (C) 2007 Elsevier Ltd. All rights reserved.