Halomonas qiaohouensis sp nov., isolated from salt mine soil in southwest China
Halomonas qiaohouensis sp nov., isolated from salt mine soil in southwest China
复制标题
桥后盐单胞菌 sp.
DOI：
10.1007/s10482-014-0189-1
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发表时间：
2014-08-01
影响因子：
2.6
通讯作者：
Cui, Xiao-Long
中科院分区：
文献类型：
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作者：
Wang, Yong-Xia;Xiao, Wei;Cui, Xiao-Long
从中国西南部云南省桥后盐矿分离得到两株革兰氏染色阴性、中度嗜盐、需氧、能运动的细菌，命名为菌株YIM QH88T 和YIM QH103。观察到该菌株的细胞呈棒状并产生奶油色的菌落。在 10–45°C（最佳 25–37°C）、pH 6.0–10.0（最佳 7.0–8.0）和 0.5–20%（w/v） NaCl（最佳 2–6%）存在下观察两种菌株的生长。发现这两个菌株含有总和特征 8 (C18:1 ω7c/ω6c)、C19:0 环 ω8c 和 C16:0 作为主要细胞脂肪酸。极性脂质被鉴定为二磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺和未知磷脂。菌株 YIM QH88T 和 YIM QH103 基因组 DNA 的 G+C 含量分别为 64.6 和 64.2 mol%，检测到的主要呼吸醌为泛醌 9。基于 16S rRNA 基因序列的系统发育分析表明，菌株 YIM QH88T 和 YIM QH103 在盐单胞菌属中形成了独特的谱系，并且是最重要的。与潘氏盐单胞菌 DSM 9661T 密切相关，16S rRNA 序列相似性分别为 97.3% 和 97.5%。菌株 YIM QH88T 和 YIM QH103 的 DNA-DNA 杂交相关性值为 95.2 ± 0.8%。这两个菌株与系统发育密切相关的盐单胞菌属物种的模式菌株之间的 DNA-DNA 相关性水平明显低于 70%。根据系统发育分析、DNA-DNA杂交相关性、表型和化学分类学特征，菌株YIM QH88T和YIM QH103应归类为盐单胞菌属新种，命名为Halomonas qiaohouensis sp。十一月被提议。模式菌株是 YIM QH88T (=DSM 26770T =CCTCC AB 2012965T)。
Two Gram-stain negative, moderately halophilic, aerobic, motile bacteria, designated strains YIM QH88T and YIM QH103, were isolated from the Qiaohou salt mine in Yunnan, southwest China. Cells of the strains were observed to be rod-shaped and produce creamy-coloured colonies. Growth of the two strains was observed at 10–45 °C (optimum 25–37 °C), at pH 6.0–10.0 (optimum 7.0–8.0), and in the presence of 0.5–20 % (w/v) NaCl (optimum 2–6 %). The two strains were found to contain summed feature 8 (C18:1 ω7c/ω6c), C19:0 cyclo ω8c and C16:0 as the major cellular fatty acids. The polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and unknown phospholipid. The G+C content of the genomic DNA of strains YIM QH88T and YIM QH103 were determined to be 64.6 and 64.2 mol%, respectively, and the predominant respiratory quinone detected was ubiquinone 9. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strains YIM QH88T and YIM QH103 formed a distinct lineage within the genus Halomonas and were most closely related to Halomonas pantelleriensis DSM 9661T with 97.3 and 97.5 % of 16S rRNA sequence similarity respectively. The DNA–DNA hybridization relatedness value for strains YIM QH88T and YIM QH103 was 95.2 ± 0.8 %. The levels of DNA–DNA relatedness between each of these two strains and the type strains of phylogenetically closely related Halomonas species were clearly below 70 %. On the basis of their phylogenetic analysis, DNA–DNA hybridization relatedness, phenotypic and chemotaxonomic characteristics, strains YIM QH88T and YIM QH103 should be classified as a novel species of the genus Halomonas, for which the name Halomonas qiaohouensis sp. nov. is proposed. The type strain is YIM QH88T (=DSM 26770T =CCTCC AB 2012965T).