Independent photoreceptive circadian clocks throughout Drosophila
Independent photoreceptive circadian clocks throughout Drosophila
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DOI：
10.1126/science.278.5343.1632
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发表时间：
1997-11-28
期刊：
影响因子：
56.9
通讯作者：
Kay, SA
中科院分区：
文献类型：
--
作者：
Plautz, JD;Kaneko, M;Kay, SA
转基因果蝇表达荧光素酶或由生物钟基因周期启动子驱动的绿色荧光蛋白，用于监测外植头、胸和腹部组织的生物钟。这些组织（包括腿和翅膀的感觉刚毛）表现出有节奏的生物发光，并且这种节律可以被光重置。外植组织的光敏特性表明，未知的光感受器可能对生物钟的光信号转导有贡献。这些结果表明，自主的昼夜节律振荡器存在于整个身体中，它们表明果蝇的单个细胞能够支持它们自己的独立时钟。
Transgenic Drosophila that expressed either luciferase or green fluorescent protein driven From the promoter of the clock gene period were used lo monitor the circadian clock in explanted head, thorax, and abdominal tissues. The tissues (including sensory bristles in the leg and wing) showed rhythmic bioluminescence, and the rhythms could be reset by light, The photoreceptive properties of the explanted tissues indicate that unidentified photoreceptors are likely to contribute to photic signal transduction to the clock. These results show that autonomous circadian oscillators are present throughout the body, and they suggest that individual cells in Drosophila are capable of supporting their own independent clocks.