Electrolytic Inferior Vena Cava Model (EIM) of Venous Thrombosis
Electrolytic Inferior Vena Cava Model (EIM) of Venous Thrombosis
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DOI：
10.3791/2737
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发表时间：
2011-07-01
影响因子：
1.2
通讯作者：
Myers, Daniel D.
中科院分区：
文献类型：
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作者：
Diaz, Jose A.;Wrobleski, Shirley K.;Myers, Daniel D.
动物模型在深静脉血栓 (DVT) 研究中发挥着至关重要的作用，以研究血栓形成、血栓溶解并测试潜在的治疗化合物 (1)。目前正在开发用于治疗和预防 DVT 的新化合物。将潜在的治疗性拮抗剂化合物递送至受影响的血栓形成的静脉一直是个问题。在治疗应用的背景下，最近建立了一种使用部分停滞并在主静脉内持续产生血栓的模型。电解下腔静脉模型 (EIM) 是 DVT 小鼠模型，允许在连续血流存在的情况下形成血栓。该模型允许治疗剂以动态方式与血栓接触，并且比其他 DVT 模型更敏感 (1)。此外，该血栓形成模型密切模拟血栓形成的临床情况，非常适合研究静脉内皮细胞活化、白细胞迁移、静脉血栓形成和测试治疗应用 (1)。 EIM 模型技术简单，易于重复，可产生一致的血栓尺寸，并可用于分析目的所需的大样本（即血栓和静脉壁）。
Animal models serve a vital role in deep venous thrombosis (DVT) research in order to study thrombus formation, thrombus resolution and to test potential therapeutic compounds (1). New compounds to be utilized in the treatment and prevention of DVT are currently being developed. The delivery of potential therapeutic antagonist compounds to an affected thrombosed vein has been problematic. In the context of therapeutic applications, a model that uses partial stasis and consistently generates thrombi within a major vein has been recently established. The Electrolytic Inferior vena cava Model (EIM) is mouse model of DVT that permits thrombus formation in the presence of continuous blood flow. This model allows therapeutic agents to be in contact with the thrombus in a dynamic fashion, and is more sensitive than other models of DVT (1). In addition, this thrombosis model closely simulates clinical situations of thrombus formation and is ideal to study venous endothelial cell activation, leukocyte migration, venous thrombogenesis, and to test therapeutic applications (1). The EIM model is technically simple, easily reproducible, creates consistent thrombi sizes and allows for a large sample (i.e. thrombus and vein wall) which is required for analytical purposes.