Efficient gene delivery into mammalian cells by recombinant baculovirus containing a hybrid cytomegalovirus promoter/Semliki Forest virus replicon
Efficient gene delivery into mammalian cells by recombinant baculovirus containing a hybrid cytomegalovirus promoter/Semliki Forest virus replicon
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通过含有杂合巨细胞病毒启动子/塞姆利基森林病毒复制子的重组杆状病毒将基因有效递送至哺乳动物细胞
DOI：
10.1002/jgm.1390
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发表时间：
2009-11-01
影响因子：
3.5
通讯作者：
Xiao, Shaobo
中科院分区：
文献类型：
--
作者：
Pan, Yongfei;Zhao, Qian;Xiao, Shaobo
杆状病毒被广泛用作昆虫细胞中生产重组蛋白的优良工具，最近已成为哺乳动物细胞的一种新颖且有吸引力的基因传递载体。甲病毒，如塞姆利基森林病毒（SFV），由于其自我复制特性，作为表达载体也受到了相当多的关注。在本研究中，我们研究了包含杂合巨细胞病毒（CMV）启动子/SFV复制子的重组杆状病毒的特征。
Baculovirus, which is widely utilized as an excellent tool for the production of recombinant protein in insect cells, has recently emerged as a novel and attractive gene delivery vehicle for mammalian cells. Alphavirus, such as Semliki Forest virus (SFV), has also received considerable attention for use as expression vectors because of its self‐replicating property. In the present study, we investigated the characterization of recombinant baculovirus incorporating a hybrid cytomegalovirus (CMV) promoter/SFV replicon.