Evolution of the trappin multigene family in the Suidae
Evolution of the trappin multigene family in the Suidae
复制标题
DOI：
10.1093/oxfordjournals.jbchem.a022140
复制
发表时间：
1998-09-01
影响因子：
2.7
通讯作者：
Hirose, S
中科院分区：
文献类型：
--
作者：
Furutani, Y;Kato, A;Hirose, S
Trappins是一类具有锚定结构域的分泌性蛋白质，其基因尤其是猪的基因经过了快速进化，产生了具有广谱作用的Trappins。为了了解这一快速进化的多基因家族的进化，我们利用聚合酶链反应（PCR）技术分离了偶蹄目动物（包括猪、疣猪、野猪、河马和牛）的trappin基因，其中两个新分离的基因是trappin-1（SPAI）和trappin-2（elafin）的直向同源物，另外两个是trappin家族的新成员，命名为trappins-6 - 11，系统发育分析表明，猪的trappin多基因家族成员是在猪科分化后通过基因重复产生的（猪和疣猪）和Tayassuidae（collared peccary），与精囊凝固蛋白（REST）和含WAP基序的蛋白质的基因结构相似性表明，陷阱蛋白是通过祖先REST和WAP基序之间的外显子改组产生的天然存在的融合蛋白。编码基因
Trappins are a group of secretory proteins containing a WAP motif with an anchoring domain, Previous studies showed that their genes, especially those of pig, have undergone rapid evolution, which produced trappins with a broad spectrum of actions. To understand the evolution of such a rapidly evolving multigene family, we isolated trappin genes of the Artiodactyla, including pig, wart hog, collared peccary, hippopotamus, and cow, by means of polymerase chain reaction (PCR), Two genes newly isolated from wart hog are orthologs of trappin-1 (SPAI) and trappin-2 (elafin), the others are novel members of the trappin family and named trappins-6 to 11, The divergence of the sequences is greatest in the region that encodes the reactive site, and intron sequences appear to be more highly conserved than the protein-coding sequences, especially among the pig paralogs, Phylogenetic analysis showed that the trappin multigene family members of pig were generated through gene duplication after the divergence of the Suidae (pig and wart hog) and Tayassuidae (collared peccary), Similarities in the gene structure with seminal vesicle clotting proteins (REST) and WAP motif-containing proteins suggest that trappins are naturally occurring fusion proteins created through exon shuffling between ancestral REST and WAP motif-coding genes.