The increased expression of miR-146 predicts poor prognosis of immune thrombocytopenia.
The increased expression of miR-146 predicts poor prognosis of immune thrombocytopenia.
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miR-146 表达增加预示着免疫性血小板减少症的不良预后。
DOI：
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
Zhigang Lu
中科院分区：
文献类型：
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作者：
Lan Deng;Ling Jiang;Kuo-Fu Tseng;Xiuju Wang;Yuan Liu;Xing Zhang;Ruihong Dong;Yuhua Li;Zhigang Lu
背景：先前的研究表明，来自正常脐带血的巨核细胞在使用或不使用免疫性血小板减少症（ITP）血浆培养时表现出一些差异。与 ITP 血浆孵育后，巨核细胞中的 MicroRNA-146 水平显着增加。方法：分析miR-146在ITP中的预后价值。血浆样本采集自 56 名 ITP 病例和 30 名健康个体。从 56 名 ITP 患者和 15 名骨髓捐赠者的骨髓中分离出巨核细胞。采用实时定量 PCR 测量血浆和巨核细胞中 miR-146 的表达。还评估了 miR-146 水平与 ITP 患者临床病理特征之间的相关性。结果：与对照值相比，ITP 患者样本中血浆和巨核细胞中的 MicroRNA-146 水平显着升高。此外，miR-146的高表达与血小板计数、淋巴细胞计数、类固醇反应、感染率和内脏出血率密切相关。结论：结果表明 miR-146 上调可能作为预测 ITP 患者不良预后的新分子标志物。
Background: Previous studies showed that megakaryocytes from normal umbilical cord blood displayed some differences when cultured with or without immune thrombocytopenia (ITP) plasma. MicroRNA-146 level in megakaryocytes increased signi cantly after incubation with ITP plasma. Methods: The prognostic value of miR-146 in ITP was analyzed. Plasma samples were collected from 56 ITP cases and 30 healthy individuals. Megakaryocytes were isolated from bone marrow obtained from the 56 ITP patients and 15 bone marrow donors. Real-time quantita- tive PCR was employed to measure miR-146 expression in plasma and megakaryocytes. The co-relations between miR-146 level and clinicopathological characteristics of ITP patients were also evaluated. Results: MicroRNA-146 levels in plasma and megakaryocytes were signi cantly higher in samples from ITP patients compared with control values. What’s more, high miR-146 expression was closely correlated with platelet count, lymphocyte count, re- sponse to steroid, infection ratio and visceral hemorrhage ratio. Conclusion: Results indicate that miR-146 upregu- lation may serve as a novel molecular marker to predict unfavorable prognosis in ITP patients.