DISSOCIATION OF SINGLE-STRANDED-DNA FROM NUCLEOSOMES FOLLOWING MODIFICATION WITH ACETIC-ANHYDRIDE
DISSOCIATION OF SINGLE-STRANDED-DNA FROM NUCLEOSOMES FOLLOWING MODIFICATION WITH ACETIC-ANHYDRIDE
复制标题
DOI：
10.1016/0006-291x(84)90763-0
复制
发表时间：
1984-01-01
影响因子：
3.1
通讯作者：
PALACIAN, E
中科院分区：
文献类型：
--
作者：
JORDANO, J;MONTERO, F;PALACIAN, E
在低离子强度（< 0.1 M NaCl）条件下，对鸡红细胞核小体进行乙酸酐修饰，形成残留颗粒，释放游离DNA。该DNA经热变性、核酸酶S1消化和羟基磷灰石洗脱鉴定为单链DNA。如果在0.6 M NaCl下进行修饰，则释放的DNA以双链为主。在修饰过程中，Lys-DNA相互作用减弱，释放了储存在折叠核小体DNA中的自由能，这可能是在低离子强度下观察到DNA变性的原因。
Modification with acetic anhydride of nucleosomes from chicken erythrocytes at low ionic strength (< 0.1 M NaCl) is accompanied by the formation of residual particles and the release of free DNA. This DNA was identified as single-stranded by thermal denaturation, digestion with nuclease S1 and elution from hydroxyapatite. If modification takes place at 0.6 M NaCl, the liberated DNA is mainly double-stranded. The release of the free energy stored in folded nucleosomal DNA, triggered by the weakening of Lys-DNA interactions which takes place upon modification, might be responsible for the observed denaturation of DNA at low ionic strength.