An arsenic(III)-oxidizing bacterial population: selection, characterization, and performance in reactors
An arsenic(III)-oxidizing bacterial population: selection, characterization, and performance in reactors
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DOI：
10.1046/j.1365-2672.2002.01726.x
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发表时间：
2002-01-01
影响因子：
4
通讯作者：
Baranger, P
中科院分区：
文献类型：
--
作者：
Battaglia-Brunet, F;Dictor, MC;Baranger, P
目的：筛选一种自养型砷（III）氧化菌群CASO1，并评价其在反应器中的性能。方法与结果：采用不含有机底物的含As（III）介质从采矿环境中筛选CASO1。在间歇和连续条件下对As（III）氧化进行了研究。利用分子生物学工具对CASO1中存在的主要生物进行了鉴定。CASO1在pH值3 ~ 8之间表现出显著的As（III）氧化活性。最佳温度是25度。在1000 mg l(-1) As（III）的存在下，仍观察到As（III）的氧化。在连续培养模式下，As（III）氧化速率达到160 mg l(-1) h（-1）。CASO1联合体包含至少两种生物体-菌株b3，它在系统发育上接近于Ralstonia picketii，菌株b6，它与硫单胞菌属有关。b6与最近亲缘生物的16S rDNA序列差异为5.9%，表明b6可能是一个新种。结论：在没有有机营养供给的情况下，利用来自采矿环境的细菌群体可以获得高的As（III）氧化活性。研究的意义和影响：CASO1种群对亚砷酸盐的生物氧化对砷污染废物或地下水的去污特别感兴趣。
Aims: To select an autotrophic arsenic(III)-oxidizing population, named CASO1, and to evaluate the performance of the selected bacteria in reactors.Methods and Results: An As(III)-containing medium without organic substrate was used to select CASO1 from a mining environment. As(III) oxidation was studied under batch and continuous conditions. The main organisms present in CASO1 were identified with molecular biology tools. CASO1 exhibited significant As(III)-oxidizing activity between pH 3 and 8. The optimum temperature was 25degreesC. As(III) oxidation was still observed in the presence of 1000 mg l(-1) As(III). In continuous culture mode, the As(III) oxidation rate reached 160 mg l(-1) h(-1) . The CASO1 consortium contains at least two organisms - strain b3, which is phylogenetically close to Ralstonia picketii , and strain b6, which is related to the genus Thiomonas . The divergence in 16S rDNA sequences between b6 and the closest related organism was 5.9%, suggesting that b6 may be a new species.Conclusions: High As(III)-oxidizing activity can be obtained without organic nutrient supply, using a bacterial population from a mining environment.Significance and Impact of the Study: The biological oxidation of arsenite by the CASO1 population is of particular interest for decontamination of arsenic-contaminated waste or groundwater.