Mechanisms of cardiac nerve sprouting after myocardial infarction in dogs
Mechanisms of cardiac nerve sprouting after myocardial infarction in dogs
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DOI：
10.1161/01.res.0000133678.22968.e3
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发表时间：
2004-07-09
影响因子：
20.1
通讯作者：
Chen, PS
中科院分区：
文献类型：
--
作者：
Zhou, SM;Chen, LS;Chen, PS
心肌梗死后心脏神经出芽和交感神经支配亢进均与心肌梗死的发生和猝死有关。然而，心肌梗死后神经出芽的机制尚不清楚。在MI后30分钟至1个月的几个时间点（30分钟至1个月），研究了梗死部位、非梗死左心室游离壁（LVFW）和左星状神经节（LSG）神经生长因子（NGF）、生长相关蛋白43（GAP 43）和其他神经标记物的表达。还测定了经心（冠状窦和主动脉之间的差异）NGF水平。急性心肌梗死导致心肌梗死后3.5小时内的经心神经生长因子浓度的立即升高，随后心脏神经生长因子和GAP 43表达的上调，这是早期和更明显的梗死部位比非梗死LVFW。然而，心脏神经发芽和交感神经支配过度在非梗死部位比梗死LVFW部位更明显，并在MI后1周达到峰值。LSG组NGF和GAP 43蛋白水平从第3天开始显著升高（P
Cardiac nerve sprouting and sympathetic hyperinnervation after myocardial infarction (MI) both contribute to arrhythmogenesis and sudden death. However, the mechanisms responsible for nerve sprouting after MI are unclear. The expression of nerve growth factor (NGF), growth associated protein 43 (GAP43), and other nerve markers were studied at the infarcted site, the noninfarcted left ventricle free wall (LVFW), and the left stellate ganglion (LSG) at several time points (30 minutes to 1 month) after MI. Transcardiac ( difference between coronary sinus and aorta) NGF levels were also assayed. Acute MI resulted in the immediate elevation of the transcardiac NGF concentration within 3.5 hours after MI, followed by the upregulation of cardiac NGF and GAP43 expression, which was earlier and more pronounced at the infarcted site than the noninfarcted LVFW. However, cardiac nerve sprouting and sympathetic hyperinnervation were more pronounced in the noninfarcted than the infarcted LVFW site and peaked at 1 week after MI. The NGF and GAP43 protein levels significantly increased in the LSG from 3 days (P