Enrofloxacin assay validation and pharmacokinetics following a single oral dose in chickens
Enrofloxacin assay validation and pharmacokinetics following a single oral dose in chickens
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DOI：
10.1111/j.1365-2885.2006.00755.x
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发表时间：
2006-10-01
影响因子：
1.3
通讯作者：
Rath, S.
中科院分区：
文献类型：
--
作者：
Da Silva, R. G.;Reyes, F. G. R.;Rath, S.
在雄性肉鸡 (Cobb) 中单次口服 10 mg ENRO/kg b.w 后，研究了氟喹诺酮类抗菌剂恩诺沙星 (ENRO) 的药代动力学。开发并验证了高效液相色谱-光电二极管阵列检测器 (DAD) (HPLC-DAD) 方法，用于血浆中 ENRO 及其主要代谢物环丙沙星的定量。采用阳离子-十八烷基混合柱和0.05 mol/L磷酸盐缓冲液(pH 2.5)/乙腈作为流动相进行HPLC分析。血浆样品制备包括蛋白质沉淀，然后在阳离子十八烷基混合柱上进行固相萃取。该方法经过验证，考虑了线性范围、线性、选择性、灵敏度、检测限 (LOD)、定量限 (LOQ)、日内和日间精密度和准确度。血浆中两种氟喹诺酮类药物的 LOD 和 LOQ 分别为 60 ng/mL 和 200 ng/mL。血浆浓度与时间图是两室开放模型的特征。 9 +/- 2 小时时达到最大血浆浓度 1.5 +/- 0.2 mg/mL。 ENRO 的消除半衰期和平均停留时间分别为 1.5 +/- 0.2 和 15.64 小时。浓度-时间曲线下面积计算为 35 +/- 4 mg(.)h/mL。
The pharmacokinetics of enrofloxacin (ENRO), a fluoroquinolone antimicrobial agent, was studied in male broiler chickens (Cobb) after single oral administration of 10 mg of ENRO/kg b.w. A high-performance liquid chromatography-photodiode array detector (DAD) (HPLC-DAD) method was developed and validated and used for quantitation of ENRO and its major metabolite ciprofloxacin in plasma. The HPLC analyses were carried out using a cationic-octadecyl mixed column and 0.05 mol/L phosphate buffer (pH 2.5)/acetonitrile as mobile phase. The sample preparation of plasma consisted of the precipitation of proteins followed by solid phase extraction on cationic-octadecyl mixed cartridges. The method was validated considering linear range, linearity, selectivity, sensitivity, limit of detection (LOD), limit of quantitation (LOQ), intra- and inter-day precisions and accuracy. The LOD and LOQ for both fluoroquinolones were 60 and 200 ng/mL for plasma. The plasma concentration vs. time graph was characteristic of a two-compartment open model. The maximal plasma concentration of 1.5 +/- 0.2 mg/mL was achieved at 9 +/- 2 h. The elimination half-life and the mean residence time of ENRO were 1.5 +/- 0.2 and 15.64 h, respectively. The area under the concentration-time curve was calculated as 35 +/- 4 mg(.)h/mL.