Development and evaluation of a line probe assay for rapid identification of pncA mutations in pyrazinamide-resistant Mycobacterium tuberculosis strains
Development and evaluation of a line probe assay for rapid identification of pncA mutations in pyrazinamide-resistant Mycobacterium tuberculosis strains
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DOI：
10.1128/jcm.00352-07
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发表时间：
2007-09-01
影响因子：
9.4
通讯作者：
Kirikae, Teruo
中科院分区：
文献类型：
--
作者：
Sekiguchi, Jun-Ichiro;Nakamura, Tomohiko;Kirikae, Teruo
结核分枝杆菌对吡嗪酰胺(PZA)的耐药性主要来自PNC，4基因的突变。我们建立了一种基于寡核苷酸探针的反向杂交线性探针方法，用于检测PNC的突变，4.对258株临床分离的结核分枝杆菌进行了PZA耐药性的检测。该方法检测PZA耐药性的敏感性和特异性均为100%，与常规PZA药敏试验结果一致。这种检测方法可以用来检测肺结核患者的痰。它似乎是可靠的和广泛适用的，鉴于其简单和快速的性能，将是一种宝贵的诊断工具。
Resistance of Mycobacterium tuberculosis to pyrazinamide (PZA) derives mainly from mutations in the pnc,4 gene. We developed a reverse hybridization-based line probe assay with oligonucleotide probes designed to detect mutations in pnc,4. The detection of PZA resistance was evaluated in 258 clinical isolates of M. tuberculosis. The sensitivity and specificity of PZA resistance obtained by this new assay were both 100%, consistent with the results of conventional PZA susceptibility testing. This assay can be used with sputa from tuberculosis patients. It appears to be reliable and widely applicable and, given its simplicity and rapid performance, will be a valuable tool for diagnostic use.