Near-Infrared-Enhanced Dual Enzyme-Mimicking Ag-TiO2-x@Alginate Microspheres with Antibactericidal and Oxygeneration Abilities to Treat Periodontitis.
Near-Infrared-Enhanced Dual Enzyme-Mimicking Ag-TiO2-x@Alginate Microspheres with Antibactericidal and Oxygeneration Abilities to Treat Periodontitis.
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DOI：
10.1021/acsami.2c17065
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发表时间：
2022-12
影响因子：
9.5
通讯作者：
Xinzhi Tan;Suru Liu;Xingyue Hu;Ruitao Zhang;Xiaofan Su;Ruojing Qian;Yao Mai;Zhao-Yun Xu
中科院分区：
文献类型：
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作者：
Xinzhi Tan;Suru Liu;Xingyue Hu;Ruitao Zhang;Xiaofan Su;Ruojing Qian;Yao Mai;Zhao-Yun Xu
彻底、持续地根除复杂牙周袋内的病原菌是治疗牙周炎的有效方法。局部缓释抗生素作为除垢和牙根规划后的补充治疗，可以持续对抗牙周袋内的细菌病原体，帮助治疗疾病，但对细菌耐药性的日益关注限制了其未来的使用。在这里，我们报道了一个基于海藻酸盐（ATA）微球封装的微型多功能Ag-TiO2-x的局部抗菌系统。研究证实，在808 nm近红外（NIR）照射下，ATA表现出强烈的光热增强的双酶模拟（过氧化物酶样和过氧化氢酶样）活性和弱的光催化活性，这可以促进低水平H2O2存在下活性氧（ROS）和O2的生成。结果表明，ATA/H2O2/NIR体系对浮游和生物膜形式的牙龈卟啉单胞菌和戈登链球菌均具有良好的抗菌活性。在活性氧的帮助下，ATA也能释放出足以抑制牙周病原体浓度的Ag+。此外，原位生成的氧气被认为可以缓解局部缺氧环境，并有助于下调脂多糖介导的牙周干细胞炎症反应。体内大鼠牙周炎治疗结果表明，ATA/H2O2/NIR系统减少了细菌负荷，缓解了炎症，促进了组织愈合。我们的工作开发了一种新的局部延长杀菌和氧合系统，用于强化牙周炎。避免使用抗生素和纳米材料，这种策略在辅助牙周炎治疗和其他生物医学应用中显示出巨大的前景。
The effective treatment for periodontitis is to completely and sustainedly eradicate the bacterial pathogens from the complex periodontal pockets. Local sustained-release antibiotics as a complementary treatment after scaling and root planning can sustainedly combat bacterial pathogens in the periodontal pockets to help treat the disease, but the increasing concern of bacterial resistance limits its future use. Here, we reported a local antibacterial system based on microsized multifunctional Ag-TiO2-x encapsulated in alginate (ATA) microspheres. We confirmed that ATA displayed strong photothermally enhanced dual enzyme-mimicking (peroxidase-like and catalase-like) activities and weak photocatalytic activity under 808 nm near-infrared (NIR) irradiation, which could boost the generation of reactive oxygen species (ROS) and O2 in the presence of low-level H2O2. As a result, the ATA/H2O2/NIR system exhibited efficient antibacterial activity against Porphyromonas gingivalis and Streptococcus gordonii in both planktonic and biofilm forms. With the help of ROS, ATA could release Ag+ in concentrations sufficient to inhibit periodontal pathogens as well. Moreover, the in situ-generated oxygen was supposed to alleviate the local hypoxic environment and would help downregulate the lipopolysaccharide-mediated inflammatory response of periodontal stem cells. The in vivo rat periodontitis treatment results demonstrated that the ATA/H2O2/NIR system reduced the bacterial load, relieved inflammation, and improved tissue healing. Our work developed a new local prolonged bactericidal and oxygenation system for enhanced periodontitis. Avoiding the usage of antibiotics and nanomaterials, this strategy showed great promise in adjunctive periodontitis treatment and also in other biomedical applications.