Subfamily I Treponema pallidum repeat protein family:: sequence variation and immunity
Subfamily I Treponema pallidum repeat protein family:: sequence variation and immunity
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DOI：
10.1016/j.micinf.2004.04.001
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发表时间：
2004-07-01
影响因子：
5.8
通讯作者：
Van Voorhis, WC
中科院分区：
文献类型：
--
作者：
Sun, ES;Molini, BJ;Van Voorhis, WC
在梅毒螺旋体亚种中发现了一个由12个成员组成的梅毒螺旋体重复蛋白家族。梅毒，梅毒的病原体。亚家族I的Tpr蛋白(C、D、F和I)在N-末端和C-末端以及区分成员的中心区域具有保守序列。这些蛋白可能在梅毒感染时的免疫反应和保护性免疫中起重要作用。在不同的梅毒分离株感染过程中，观察到了对亚家族I Tpr蛋白的强烈抗体反应。在梅毒T亚种中还发现了一个亚家族I Tpr成员TprD的序列变异。梅毒分离株。在这项研究中，我们检查了菌株之间剩余的亚家族I Tpr蛋白的序列。Tprf和TprI在梅毒T亚种中都是保守的。梅毒分离株。而TprC则表现出一定的异质性。本文进一步研究了TPRF蛋白的免疫应答和保护作用。我们证明了亚家族I Tpr蛋白的N端保守区在感染过程中能引起强烈的抗体和T细胞反应。该区域的免疫可减轻感染攻击时梅毒病变的发展。(C)2004年爱思唯尔公司。版权所有。
A 12-membered Treponema pallidum repeat (Tpr) protein family has been identified in T pallidum subsp. pallidum, the causative agent of syphilis. The subfamily I Tpr proteins (C, D, F and I) possess conserved sequence at the N- and C-termini and central regions that differentiate the members. These proteins may be important in the immune response during syphilis infection and in protective immunity. Strong antibody responses have been observed toward some of the subfamily I Tpr proteins during infection with different syphilis isolates. Some sequence variation has also been identified in one subfamily I Tpr member, TprD, among T pallidum subsp. pallidum isolates. In this study, we examined sequences in the remaining subfamily I Tpr proteins among strains. Both TprF and TprI were conserved among T pallidum subsp. pallidum isolates. While some heterogeneity was identified in TprC. We further examined the immune response and protective capacity of TprF protein in this paper. We demonstrate that the N-terminal conserved region of the subfamily I Tpr proteins elicits strong antibody and T-cell responses during infection. and immunization with this region attenuates syphilitic lesion development upon infectious challenge. (C) 2004 Elsevier SAS. All rights reserved.