Cough and expiration reflexes elicited by inhaled irritant gases are intensified in ovalbumin-sensitized mice
Cough and expiration reflexes elicited by inhaled irritant gases are intensified in ovalbumin-sensitized mice
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DOI：
10.1152/ajpregu.00444.2016
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发表时间：
2017-05-01
影响因子：
2.8
通讯作者：
Lee, Lu-Yuan
中科院分区：
文献类型：
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作者：
Zhang, Cheng;Lin, Ruei-Lung;Lee, Lu-Yuan
本研究旨在确定卵清蛋白 (Ova) 主动致敏对小鼠吸入刺激性气体的咳嗽反应的影响。清醒的小鼠在记录室内自由活动，同时记录记录室内的压力变化和小鼠运动的音频和视频信号，以测量咳嗽反射（CR）和呼气反射（ER）的频率。为了进一步验证咳嗽分析的准确性，还通过手术植入一组小鼠胸膜内的遥测传感器记录了胸膜内压力。在刺激性气体吸入挑战期间，二氧化硫（SO2；200 和 400 ppm）或氨（NH3；0.1% 和 0.2%）以恒定流速吸入室内 8 分钟。在不同组的小鼠中进行为期 25 天的卵子致敏和使用媒介物 (Veh) 的假致敏。我们的结果表明，1）SO2 和 NH3 吸入挑战在 Ova 致敏之前以浓度依赖性方式增加 CR 和 ER 频率； 2）Ova致敏后基线CR频率显着升高，并伴有明显的气道炎症； 3) Ova 致敏也显着增强了 CR 和 ER 对 SO2 和 NH3 吸入挑战的反应；与此形成鲜明对比的是，Veh 组在假致敏后咳嗽反应没有变化。总之，Ova 致敏导致基线咳嗽频率明显且持续增加，并且还增强了对吸入刺激性气体的 CR 和 ER 反应，这可能是由于过敏性炎症引起的气道感觉神经过敏所致。
This study was designed to determine the effect of active sensitization with ovalbumin (Ova) on cough responses to inhaled irritant gases in mice. Conscious mice moved freely in a recording chamber, while the pressure change in the chamber and audio and video signals of the mouse movements were recorded simultaneously to measure the frequencies of cough reflex (CR) and expiration reflex (ER). To further verify the accuracy of cough analysis, the intrapleural pressure was also recorded by a telemetry sensor surgically implanted in the intrapleural space in a subgroup of mice. During the irritant gas inhalation challenge, sulfur dioxide (SO2; 200 and 400 ppm) or ammonia (NH3; 0.1% and 0.2%) was drawn into the chamber at a constant flow rate for 8 min. Ova sensitization and sham sensitization with vehicle (Veh) were performed over a 25-day period in separate groups of mice. Our results showed that 1) both SO2 and NH3 inhalation challenges increased CR and ER frequencies in a concentration-dependent manner before Ova sensitization; 2) the baseline CR frequency was significantly elevated after Ova sensitization, accompanied by pronounced airway inflammation; and 3) Ova sensitization also markedly augmented the responses of CR and ER to both SO2 and NH3 inhalation challenges; in sharp contrast, the cough responses did not change after sham sensitization in the Veh group. In conclusion, Ova sensitization caused distinct and lingering increases in baseline cough frequency, and also intensified both CR and ER responses to inhaled irritant gases, which probably resulted from an allergic inflammation-induced hypersensitivity of airway sensory nerves.