A mouse model of proliferative vitreoretinopathy induced by dispase
A mouse model of proliferative vitreoretinopathy induced by dispase
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DOI：
10.1006/exer.2002.2031
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发表时间：
2002-11-01
影响因子：
3.4
通讯作者：
Suburo, AM
中科院分区：
文献类型：
--
作者：
Soler, MVC;Gallo, JE;Suburo, AM
用检眼镜和组织化学方法研究了C57 BL/6J小鼠玻璃体内注射分散酶诱导的增殖性玻璃体视网膜病变样疾病。在注射后1、2、4、6和8周通过检眼镜检查对玻璃体内出血、玻璃体内斑点、视网膜褶皱和视网膜前膜的频率进行评分。玻璃体内斑点对应于表现出F4/80免疫反应性（巨噬细胞/小胶质细胞标记物）的游离细胞。视网膜皱褶总是出现在视网膜前膜可以观察到。注射Dispase的眼总是比注射盐水的眼显示出更高的皱褶和膜的频率，在玻璃体内出血的情况下，皱褶和膜比在没有玻璃体内出血的情况下出现得更早，更广泛。Muller视网膜细胞的胶质细胞酸性蛋白免疫反应性有明显变化。这是不存在于正常的穆勒细胞，但在分散酶注射的动物，它表达在放射状过程中的网站的视网膜褶皱，后来延伸到整个视网膜。这两个epi-和视网膜下膜包含的细胞可能来自米勒细胞，因为他们表现出共同定位的胶质细胞酸性蛋白和谷氨酰胺合成酶的免疫反应。F4/80还存在于视网膜、视网膜上膜和视网膜下膜内的许多细胞中。相比之下，视网膜色素上皮细胞标志物RPE 65仅限于视网膜下膜。可以得出结论，分散酶在小鼠中诱导了增殖性玻璃体视网膜病变样病症，其中巨噬细胞和神经胶质源性细胞的贡献很大。(C)2002年由Elsevier Science Ltd.出版
A proliferative vitreoretinopathy-like condition induced by intravitreal dispase injection in C57BL/6J mice was studied using ophthalmoscopic and histochemical procedures. The frequency of intravitreal hemorrhage, intravitreal spots, retinal folds and epiretinal membranes was scored by ophthalmoscopic examination at 1, 2 4, 6 and 8 weeks after the injection. Intravitreal spots corresponded to free cells exhibiting F4/80 immunoreactivity, a macrophage/microglial marker. Retinal folds always appeared before an epiretinal membrane could be observed. Dispase-injected eyes always showed a much higher frequency of folds and membranes than saline-injected eyes, Folds and membranes appeared earlier and were more extensive in the presence of intravitreal hemorrhage than in its absence. Muller retinal cells exhibited significant changes in glial fibrillary acidic protein-immunoreactivity. This was absent in normal Muller cells but, in dispase-injected animals, it was expressed in radial processes at the site of retinal folds, later extending to the whole retina. Both epi- and subretinal membranes contained cells probably derived from Miller cells, since they exhibited co-localization of glial fibrillary acidic protein- and glutamine synthase immunoreactivities. F4/80 was also present in numerous cells within the retina, epi- and subretinal membranes. By contrast, the retinal pigment epithelium cell marker RPE65 was restricted to subretinal membranes. It can be concluded that dispase induced a proliferative vitreoretinopathy-like condition in mice, with a strong contribution of macrophage- and glial-derived cells. (C) 2002 Published by Elsevier Science Ltd.