Effects of 5-thio-D-glucose on cellular adenosine triphosphate levels and deoxyribonucleic acid rejoining hy hypoxic and aerobic Chinese hamster cells.
Effects of 5-thio-D-glucose on cellular adenosine triphosphate levels and deoxyribonucleic acid rejoining hy hypoxic and aerobic Chinese hamster cells.
复制标题
5-硫代-D-葡萄糖对细胞三磷酸腺苷水平和脱氧核糖核酸重新加入缺氧和需氧中国仓鼠细胞的影响。
作者：
W. Nagle;A. Moss;H. G. Roberts;M. Baker
用5-硫代- d -葡萄糖（5-SH-D-Glc）处理的V79中国仓鼠细胞，在缺氧和有氧培养中测定细胞内三磷酸腺苷（ATP）水平。这种葡萄糖类似物是一种已知的d -葡萄糖运输和代谢抑制剂，在细胞代谢过程中使细胞缺氧时减少ATP，但在有氧培养物中则没有。通过碱性蔗糖梯度沉降技术测量，ATP耗尽的细胞无法重新连接x射线诱导的脱氧核糖核酸（DNA）链断裂。结果表明，5-SH-D-Glc导致依赖糖酵解产生ATP的细胞的能量代谢减少。对放射治疗的推断是，抑制葡萄糖代谢选择性地消耗缺氧细胞中的能量储备，使这些细胞对放射更敏感，从而导致更有效的肿瘤治疗。
Intracellular adenosine triphosphate (ATP) levels were measured in both hypoxic and aerobic cultures of V79 Chinese hamster cells treated with 5-thio-D-glucose (5-SH-D-Glc). This glucose analog, a known inhibitor of D-glucose transport and metabolism, reduced ATP in cell cultures allowed to become hypoxic by cell metabolism, but not in aerobic cultures treated similarly. Cells depleted of ATP were unable to rejoin x-ray-induced deoxyribonucleic acid (DNA) strand breaks as measured by the alkaline surcrose gradient sedimentation technique. Results show that 5-SH-D-Glc leads to reduced energy metabolism in cells dependent on glycolysis for ATP production. The inference for radiation therapy is that inhibition glucose metabolism selectively depletes energy reserves in hypoxic cells, rendering these cells more radiosensitive and leading to a more effective tumor treatment.