Preliminary study of the presence and association of bacteria and archaea in teeth with apical periodontitis
Preliminary study of the presence and association of bacteria and archaea in teeth with apical periodontitis
复制标题
根尖周炎牙齿中细菌和古菌的存在及其关联的初步研究。
DOI：
10.1111/j.1365-2591.2009.01639.x
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发表时间：
2009-12-01
影响因子：
5
通讯作者：
Liang, J. P.
中科院分区：
文献类型：
--
作者：
Jiang, Y. T.;Xia, W. W.;Liang, J. P.
目的应用逆转录聚合酶链式反应(RT-PCR)检测77例根管标本中细菌和古生菌的存在及其相关性。方法收集77例根管标本，其中牙髓坏死组42例(初次感染)，常规根管治疗失败35例(继发感染)。结果42例初发感染根管标本中细菌检出39例(93%)，古菌检出16例(38%)。在诊断为继发性根管感染的病例中，30/35例(86%)检出细菌，6/35例(17%)检出古生菌。在细菌呈阳性的运河中，古生菌总是与细菌结合在一起。同时检出细菌和古生菌的有症状病例(16/22，73%)明显高于单独检出细菌的患者(21/47，45%)(P<；0.05)。结论本研究证实了根管感染中同时检出古生菌的存在，并进一步将其与临床症状联系起来。这种联系的性质需要进一步研究。
P>AimTo investigate, by reverse transcription polymerase chain reaction (RT-PCR), the presence and association of bacteria and archaea in primary and secondary root canal infections.MethodologyA total of 77 root canal samples from 77 Chinese patients, 42 with necrotic pulp tissues (primary infection) and 35 with failed prior conventional root canal treatment (secondary infection), aseptically exposed at the first patient visit, were studied. Total RNA was isolated directly from each sample, and 16S rRNA gene-based RT-PCR assays were used to determine the presence of bacteria and archaea, respectively.ResultsBacteria were detected in 39/42 (93%) of root canal samples from teeth with primary infections, and archaea in 16/42 (38%). In the cases diagnosed as secondary root-infected canals, bacteria were detected in 30/35 (86%), whilst archaea were detected in 6/35 (17%) of cases. Amongst the canals, which were positive for bacteria, archaea were always found in combination with bacteria. The incidence of symptomatic cases positive for both bacteria and archaea (16/22, 73%) were significantly higher than those positive for bacteria alone (21/47, 45%) (P < 0.05).ConclusionsThis study confirms the presence of archaea in root canal infections and further implicates them in an association with clinical symptoms. The nature of this association requires further study.