A SPLICED LEADER IS PRESENT ON A SUBSET OF MESSENGER-RNAS FROM THE HUMAN PARASITE SCHISTOSOMA-MANSONI
A SPLICED LEADER IS PRESENT ON A SUBSET OF MESSENGER-RNAS FROM THE HUMAN PARASITE SCHISTOSOMA-MANSONI
复制标题
DOI：
10.1073/pnas.87.22.8879
复制
发表时间：
1990-11-01
影响因子：
11.1
通讯作者：
ROTTMAN, FM
中科院分区：
文献类型：
--
作者：
RAJKOVIC, A;DAVIS, RE;ROTTMAN, FM
我们提出的证据表明，人类寄生吸虫曼氏血吸虫的mrna子集在其5'末端含有相同的36个核苷酸剪接先导（SL）序列。SL是由90个核苷酸的非聚腺苷化RNA （SL RNA）衍生而来，可能是通过反式剪接。在锥虫原虫或线虫中，SL和SL RNA与先前描述的反式剪接先导体和SL RNA都没有显著的序列一致性。然而，一些特征，如预测的二级结构、三甲基鸟苷帽和潜在的Sm结合位点，表明在广泛不同的生物体中，SL rna具有相似性。我们的证据还表明，3-羟基-3-甲基戊二酰辅酶a还原酶基因的外显子3受体位点既可以通过反式剪接剪切到SL上，也可以通过顺式剪接剪切到上游外显子2上。在S. mansoni （platyhelmintes门的一员）中存在SL序列，表明反式剪接可能是其他低等无脊椎动物的共同特征。
We present evidence that a subset of mRNAs in the human parasitic trematode Schistosoma mansoni contain an identical 36-nucleotide spliced leader (SL) sequence at their 5'' termini. The SL is derived from a 90-nucleotide nonpolyadenylylated RNA (SL RNA), presumably by trans-splicing. Neither the SL nor the SL RNA share significant sequence identity with previously described trans-spliced leaders and SL RNAs in trypanosomatid protozoans or nematodes. However, several features, such as predicted secondary structure, trimethylguanosine cap, and potential Sm binding site, suggest similarities among SL RNAs in widely divergent organisms. Our evidence also indicates that the exon 3 acceptor site of the 3-hydroxy-3-methylglutaryl-CoA reductase gene can be spliced either to the SL by trans-splicing or to an upstream exon, 2, by cis-splicing. The presence of a SL sequence in S. mansoni, a member of the phylum Platyhelminthes, suggests that trans-splicing may be a common feature of other lower invertebrates.