Enhanced susceptibility to Trichuris muris infection of B10Br mice treated with the probiotic Lactobacillus casei
Enhanced susceptibility to Trichuris muris infection of B10Br mice treated with the probiotic Lactobacillus casei
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DOI：
10.1016/j.intimp.2007.10.003
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发表时间：
2008-01-01
影响因子：
5.6
通讯作者：
Bolas-Fernandez, Francisco
中科院分区：
文献类型：
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作者：
Dea-Ayuela, Ma Auxitiadora;Rama-Iniguez, Sara;Bolas-Fernandez, Francisco
本研究旨在建立一个合适的模型，以探讨益生菌与肠道蠕虫感染相互作用的免疫调节基础。在易感的B10 Br小鼠中使用小鼠鞭虫Trichuris muris。小鼠口服活的或死的益生菌L。casei感染，1周后，它们接受400个鼠毛癣菌含胚卵的攻击感染。在感染后第22天，用活菌或死菌治疗显著增加了肠道蠕虫负担。维艾博湖干酪素显著降低了由攻击感染诱导的粪便伊加水平。MNL细胞对有丝分裂原的增殖反应被死细菌显着降低，并被活细菌废除。此外，益生菌的存在与IFN-γ、TNF-α、IL-4和IL-13的显著降低相关，而对于攻击对照感染，在MNL和PP中对IL-5没有影响。L的存在。酪蛋白没有显著改变MLN和PR中CD 4（+）和CD 8（+）细胞的比例。酪蛋白与肠鞭虫鼠弓形虫感染的易感性增加有关。这一证据的机制可能与TNF-α依赖性Th 2效应物对鼠嗜热厌氧菌应答的失活有关，这是由于益生菌剂诱导的这种细胞因子的强烈下调。(C)2007 Elsevier B. V.保留所有权利。
The aim of this paper was to establish a suitable model for exploring the immunoregulatory bases of the interaction of probiotics with intestinal helminth infections. The mouse whipworm Trichuris muris, was used in the susceptible B10Br mice. Mice were treated orally with either viable or dead probiotic L. casei and 1 week latter they were submitted to a challenge infection of 400 embryonated eggs of T muris. Treatment with either viable or dead bacteria significantly increased the intestinal worm burden by day 22 post-infection. Viable L. casei significantly reduced the levels of faecal IgA induced by challenge infection. The proliferation response of MNL cells against mitogens was significantly reduced by dead bacteria and abrogated by viable bacteria. Furthermore the presence of the probiotic was associated to a significant decrease in IFN-gamma, TNF-alpha, IL-4 and IL-13 with no effect on IL-5 in both MNL and PP with regard to challenge control infection. The presence of L. casei did not significantly modify the proportion of CD4(+) and CD8(+) cells in both MLN and PR In summary, in the susceptible B10Br mouse strain the presence of probiotic L. casei is associated to an increased susceptibility to infection by the intestinal whipworm T muris. The mechanisms underlying this evidence could be related to the deactivation of TNF-alpha dependent Th2 effector responses against T muris due to the strong down-regutation of this cytokine that is induced by the probiotic agent. (C) 2007 Elsevier B.V. All rights reserved.