Triple immunofluorescent labeling of FtsZ, dynamin, and EF-Tu reveals a loose association between the inner and outer membrane mitochondrial division machinery in the red alga Cyanidioschyzon merolae
Triple immunofluorescent labeling of FtsZ, dynamin, and EF-Tu reveals a loose association between the inner and outer membrane mitochondrial division machinery in the red alga Cyanidioschyzon merolae
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DOI：
10.1369/jhc.4c6315.2004
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发表时间：
2004-07-01
影响因子：
3.2
通讯作者：
Kuroiwa, T
中科院分区：
文献类型：
--
作者：
Nishida, K;Misumi, O;Kuroiwa, T
在原始真核生物的线粒体中，FtsZ和发动蛋白分别是参与内膜和外膜分裂的机器的一部分。这些基因在叶绿体分裂过程中也通常以相同的方式发挥作用。在这项研究中，直接的内部和外部的分裂机制的本地化之间的关系是第一次。利用窄带滤光片和明亮的光稳定染料对红腹蓝腹菌进行了三重免疫荧光标记。FtsZ（CmFtsZ 1）和发动蛋白（CmDnm 1）的定位在整个线粒体分裂周期中用另一种线粒体标记蛋白（线粒体翻译延伸因子EF-Tu）同时进行了检查，其定位也被证明与线粒体基质相同。FtsZ和发动蛋白不一定共定位时，都被招募到线粒体收缩部位，这表明内部和外部的分裂机制是不紧密的协会在分裂的后期阶段。
In the mitochondria of primitive eukaryotes, FtsZ and dynamin are part of the machinery involved in division of the inner and outer membranes, respectively. These genes also commonly function in the same manner during chloroplast division. In this study, a relationship between the localization of the inner and outer division machinery was directly shown for the first time. Triple immunofluorescent labeling was performed in the red alga Cyanidioschyzon merolae by a device using narrow bandpass filter sets and bright photostable dyes. FtsZ (CmFtsZ1) and dynamin (CmDnm1) localizations were examined simultaneously throughout the mitochondrial division cycle with an alternative mitochondrial marker protein, the mitochondrial translation elongation factor EF-Tu, whose localization was also shown to be identical to the mitochondrial matrix. FtsZ and dynamin did not necessarily co-localize when both were recruited to the mitochondrial constriction site, indicating that inner and outer dividing machineries are not in tight association during the late stage of division.