Colonisation pattern of nonpathogenic Fusarium oxysporum, a potential biological control agent, in roots and rhizomes of tissue cultured Musa plantlets
Colonisation pattern of nonpathogenic Fusarium oxysporum, a potential biological control agent, in roots and rhizomes of tissue cultured Musa plantlets
复制标题
非致病性尖孢镰刀菌（一种潜在的生物防治剂）在组织培养芭蕉苗的根和根茎中的定殖模式
DOI：
10.1111/j.1744-7348.2006.00064.x
复制
发表时间：
2006
期刊：
影响因子：
--
通讯作者：
D. Coyne
中科院分区：
文献类型：
--
作者：
P. Paparu;T. Dubois;C. Gold;B. Niere;E. Adipala;D. Coyne
在实验室条件下，非致病性内生尖孢镰刀菌对香蕉象鼻虫和线虫造成高死亡率。在香蕉（Musa spp）组织培养植株上接种后，成功定殖是有效生物防治这些害虫的必要条件。研究了两株非致病性乌干达尖孢镰刀菌（V2w2和III4w1）的根和根茎定植模式。光镜下对Nabusa （AAA-EA）进行了研究。根茎中的定植百分率（93%）高于根中的定植百分率（56%），但根中的菌丝密度（0.30 mm⁻²）高于根茎中的菌丝密度（0.21 mm⁻²）。根底的定殖率（76%）高于根中部（53%）或根尖（39%）。这两种菌株都在根和根茎上定植，许多菌丝感染皮下组织，但感染皮层的菌丝较少。未记录维管组织的定植。尽管在根和根茎的细胞间隙和细胞内存在菌丝，但细胞结构正常。我们的报告提供了香蕉内生菌定植的首次原位观察和定量。研究表明，接种后，尖孢F. oxysporum菌株V2w2和III4w1能够穿透完整的寄主组织并在寄主内部重新定殖，这是其适合作为生物防治剂的重要一步。©2006作者。
Under laboratory conditions, nonpathogenic, endophytic Fusarium oxysporum inflicts high mortality among banana weevils and nematodes. Following inoculation into banana (Musa spp.) tissue cultured plants, successful colonisation is necessary for efficient biological control of these pests. The pattern of root and rhizome colonisation by two nonpathogenic Ugandan F. oxysporum strains (V2w2 and III4w1) in cv. Nabusa (AAA-EA) was investigated using light microscopy. Percentage of colonisation in the rhizomes (93%) was higher than in the roots (56%), but hyphal density in the roots (0.30 mm ⁻²) was higher than in the rhizomes (0.21 mm⁻²). The root bases were better colonised (76%) than root midsections (53%) or tips (39%). Both the strains colonised the roots and the rhizomes, with numerous hyphae infecting the hypodermis but fewer infecting the cortex. Colonisation of vascular tissues was not recorded. Despite the presence of hyphae in intercellular and intracellular spaces of the roots and the rhizomes, normal cell structure was observed. Our report provides the first in situ observation and quantification of endophyte colonisation in banana. The study demonstrated the ability of F. oxysporum strains V2w2 and III4w1 to penetrate intact host tissues and recolonise the host internally upon inoculation, an important step for their suitability as biological control agents. © 2006 The Authors.