Premolt biomarker metabolite N－acetylglucosamino－ 1,5－lactone in female urine of the helmet crab Telmessus cheiragonus.
Premolt biomarker metabolite N－acetylglucosamino－ 1,5－lactone in female urine of the helmet crab Telmessus cheiragonus.
复制标题
盔甲蟹 Telmessus cheiragonus 雌性尿液中蜕皮前生物标志物代谢物 N－乙酰氨基葡萄糖－ 1,5－内酯。
DOI：
10.1086/bblv230n2p143
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发表时间：
2016
影响因子：
1.6
通讯作者：
Hiroshi Nagai
中科院分区：
文献类型：
--
作者：
Hirona Yano;Michiya Kamio;Hiroshi Nagai
N-乙酰氨基葡萄糖-1，5-内酯(NAGL)是青蟹蜕皮的生物标志物。这种化合物在尿液中的浓度在蜕皮前阶段最高。由于性成熟的未蜕皮雌性在尿液中释放性信息素，NAGL是候选性信息素分子公司。萨皮德斯。该化合物在其他物种中未见报道。在本研究中，我们用核磁共振波谱对头盔蟹尿中NAGL的浓度进行了定量，发现其浓度在蜕皮当天增加，在蜕皮后下降。但由于蜕皮前尿量最低，蜕皮后2~5天尿量有所增加，所以在蜕皮后2~5天，个体采集的NAGL总量最大。Nagl int的最高中位浓度。CheiraGonas为29μ摩尔L−1，是已报道的最高浓度的75%。萨皮德斯。这是首次报道Nagl作为蜕皮生物标记物存在于除C。萨皮德斯。我们假设Nagl是这两个物种信息素花束的一部分。
N-acetylglucosamino-1,5-lactone (NAGL) is a molting biomarker in the blue crabCallinectes sapidus. The concentration of this compound in urine is highest at the premolt stage. Since sexually mature premolt females release sex pheromone in their urine, NAGL is a candidate sex pheromone molecule inC. sapidus. This compound has not been reported in other species. In the present study, we quantified the concentration of NAGL in the urine of the helmet crabTelmessus cheiragonus, using nuclear magnetic resonance spectroscopy, and found that the concentration increases toward the day of molting and decreases after molting. However, the total amount of NAGL collected from individual animals was greatest two to five days after molting, because the amount of urine collected was the lowest at the premolt stage, and it increased after molting. The highest median concentration of NAGL inT. cheiragonaswas 29 μmol l−1, which is 75% of the highest concentration reported inC. sapidus. This is the first report of NAGL as a molting biomarker in a species other thanC. sapidus. We assume that NAGL is part of a pheromone bouquet in these two species.