Cytokine gene expression by peripheral blood leukocytes in dogs experimentally infected with a new virulent strain of Ehrlichia canis
Cytokine gene expression by peripheral blood leukocytes in dogs experimentally infected with a new virulent strain of Ehrlichia canis
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DOI：
10.1196/annals.1374.090
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发表时间：
2006-01-01
期刊：
影响因子：
--
通讯作者：
Rikihisa, Yasuko
中科院分区：
文献类型：
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作者：
Unver, Ahmet;Huang, Haibin;Rikihisa, Yasuko
犬埃利希体（E. canis）是一种缺乏脂多糖（LPS）的专性细胞内细菌，可引起犬单核细胞埃利希体病，这是一种伴有血液学异常的慢性发热性疾病。本研究使用实时 RT-PCR 分析了实验性感染新的犬埃里希毒株的狗的外周血白细胞中 IL-1β、IL-2、IL-6、IL-8、IFN-γ 和 TNF-α mRNA 的时间表达水平。从接种后 2 天到 52 天，所有三只受感染的狗中，由 P-肌动蛋白转录物水平标准化的 IL-1 β 和 IL-8 转录物的相对水平显着上调，而 TNF-α 和 IFN-γ 转录物的相对水平仅微弱上调。与阳性对照（ConA刺激的犬外周血白细胞）相比，IL-2和IL-6基因的表达极低。这项研究表明，犬埃立克体可以诱导促炎细胞因子基因子集的慢性表达：这些细胞因子生成的平衡、时间和持续时间可能有助于犬埃立克体病的进展。
Ehrlichia canis (E. canis) is a lipopolysaccharide (LPS)-deficient obligatory intracellular bacterium that causes canine monocytic ehrlichiosis, a chronic febrile disease accompanied with hematological abnormality. This study analyzed temporal expression levels of IL-1 beta, IL-2, IL-6, IL-8, IFN-gamma, and TNF-alpha mRNA by peripheral blood leukocytes from dogs experimentally infected with a new virulent strain of E. canis by using real-time RT-PCR. Relative levels of IL-1 beta and IL-8 transcripts normalized by the P-actin transcript levels, were significantly upregulated, whereas those of TNF-alpha and IFN-gamma transcripts were only weakly upregulated in all three infected dogs, starting from 2 days up to 52 days post inoculation. The expressions of IL-2 and IL-6 genes were extremely low compared with the positive control (ConA-stimulated canine peripheral blood leukocytes). This study showed that E. canis can induce chronic expression of a subset of proinflammatory cytokine genes: balance, timing, and duration of these cytokine generations may contribute to the progression of canine ehrlichiosis.