Interactions among rods in the isolated retina of Bufo marinus.
Interactions among rods in the isolated retina of Bufo marinus.
复制标题
蟾蜍离体视网膜中视杆细胞之间的相互作用。
DOI：
10.1113/jphysiol.1981.sp013704
复制
发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Pinto,LH
中科院分区：
文献类型：
--
作者：
Griff,ER;Pinto,LH
1.本文分析了离体蟾蜍视网膜视杆细胞间相互作用的存在和程度。用微量移液器从外节进行细胞内记录。使用复合显微镜将精确测量几何形状的刺激传递到外节。2.为了证明相互作用的存在，同时刺穿了两个棒;电流注入一个棒导致另一个棒中出现类似符号的电流感应电位。3.当圆形刺激物的直径从30微米增加到500微米时，杆的平均灵敏度增加了1.2 +/-0.3对数单位（S.D.），尽管通过刺穿的杆测量的照度被调节为恒定。4.通过在穿刺棒的几个位移中的每一个位移处呈现暗淡的狭缝形刺激来检查相互作用的程度。这种刺激要么在聚焦于外节之前穿过视网膜（透照），要么直接聚焦于外节（入射照明）。对于每个位移，测量散射到穿刺棒上的光量和棒的响应。5.对于刺激的每个位移，评估散射到刺穿杆上的光量。对于入射照明，通过测量关于狭缝刺激的漂白颜料的分布来进行该评估。漂白可以用随距离减小的指数来描述;该指数具有4微米的空间常数。对于透照，评估是以两种方式进行的。测量穿过穿刺棒的光，发现其随狭缝位移呈指数下降;其平均空间常数为9.3 +/-3.2微米（S.D.）。在一些实验中，漂白色素的分布有关的狭缝刺激进行了测量，并发现随着距离呈指数下降，这后一个指数有一个稍长的空间常数比使用透射光测量的指数。6.对于每个刺穿杆，指数相互作用函数与围绕刺穿杆的刺激照度的测量分布的卷积可以被拟合到响应幅度与狭缝刺激的位移的曲线图。对于通过透照呈现的刺激，描述相互作用函数的平均空间常数为20 +/-6微米（S.D.）。对于由入射照明呈现的刺激，相互作用函数的平均空间常数为22 +/-4微米（S.D.）。7.本报告提出了新的证据，杆之间的相互作用存在于离体视网膜和相互作用的程度可以描述的空间常数约为20微米。
1. The existence and extent of interactions among rods were analysed in the isolated retina of Bufo marinus. Intracellular recordings were made from the outer segments with micropipettes. Stimuli of precisely measured geometry were delivered to the outer segments using a compound microscope. 2. To demonstrate the existence of interactions two rods were simultaneously impaled; current injected into one rod resulted in a current‐induced potential of like sign in the other rod. 3. When the diameter of a circular stimulus was increased from 30 to 500 micrometer the mean sensitivity of the rods increased by 1.2 +/‐ 0.3 log units (S.D.), though the illuminance measured through the impaled rod was adjusted to be constant. 4. The extent of interactions was examined by presenting a dim, slit‐shaped stimulus at each of several displacements from an impaled rod. This stimulus either passed through the retina before coming into focus on the outer segments (transillumination) or was focused directly on the outer segments (incident illumination). For each displacement both the amount of light scattered onto the impaled rod and the response of the rod were measured. 5. The amount of light scattered onto the impaled rod was assessed for each displacement of the stimulus. For incident illumination this assessment was made by measuring the distribution of bleached pigment about the slit stimulus. The bleaching could be described by an exponential that decreased with distance; this exponential had a space constant of 4 micrometer. For transillumination the assessment was made in two ways. The light that passed through the impaled rod was measured and found to decrease exponentially with slit displacement; the mean space constant of this exponentially was 9.3 +/‐ 3.2 micrometer (S.D.). In few experiments the distribution of bleached pigment about the slit stimulus was measured and was found to decrease exponentially with distance; this latter exponential had a slightly longer space constant than the exponential measured using transmitted light. 6. For each impaled rod a convolution of an exponential interaction function with the measured distribution of stimulus illuminance about the impaled rod could be fitted to the plot of response amplitude versus displacement of the slit stimulus. For stimuli presented by transillumination the mean space constant describing the interaction functions was 20 +/‐ 6 micrometer (S.D.). For stimuli presented by incident illumination the mean space constant of the interaction functions was 22 +/‐ 4 micrometer (S.D.). 7. This report presents new evidence that interactions among rods exist in the isolated retina and that the extent of interactions can be described by a space constant of about 20 micrometer.