Electro-plasmonic nanoantenna: A nonfluorescent optical probe for ultrasensitive label-free detection of electrophysiological signals
Electro-plasmonic nanoantenna: A nonfluorescent optical probe for ultrasensitive label-free detection of electrophysiological signals
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DOI：
10.1126/sciadv.aav9786
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发表时间：
2019-10-01
期刊：
影响因子：
13.6
通讯作者：
Yanik, Ahmet A.
中科院分区：
文献类型：
--
作者：
Habib, Ahsan;Zhu, Xiangchao;Yanik, Ahmet A.
利用光前所未有的时空分辨率来检测电生理信号一直是科学家近50年来的目标。然而，由于缺乏能够有效地将电活动转换为高光子计数光信号的电光转换器，实现这一目标的进展仍然难以实现。在这里，我们介绍了一种超灵敏和极亮的纳米级电场探测器，克服了现有光场记录器的低光子计数限制。我们的电浆纳米天线具有显著增强的横截面(与电压敏感型荧光染料的典型值类似于10(-2)nm(2)，而对于量子点的横截面类似于10(-2)nm(2)和类似于1 nm(2))，能够可靠地检测衍射受限光点的局部电场动态，具有极高的灵敏度和信噪比(类似于60至220)。在我们的电光实验中，我们展示了在千赫兹频率下的高时间分辨率电场测量，并实现了低强度光(11 mW/mm(2))下心肌细胞网络级电活动的无标记光学记录。
Harnessing the unprecedented spatiotemporal resolution capability of light to detect electrophysiological signals has been the goal of scientists for nearly 50 years. Yet, progress toward that goal remains elusive due to lack of electro-optic translators that can efficiently convert electrical activity to high photon count optical signals. Here, we introduce an ultrasensitive and extremely bright nanoscale electric-field probe overcoming the low photon count limitations of existing optical field reporters. Our electro-plasmonic nanoantennas with drastically enhanced cross sections (similar to 10(4) nm(2) compared to typical values of similar to 10(-2) nm(2) for voltage-sensitive fluorescence dyes and similar to 1 nm(2) for quantum dots) offer reliable detection of local electric-field dynamics with remarkably high sensitivities and signal-to-shot noise ratios (similar to 60 to 220) from diffraction-limited spots. In our electro-optics experiments, we demonstrate high-temporal resolution electric-field measurements at kilohertz frequencies and achieved label-free optical recording of network-level electrogenic activity of cardiomyocyte cells with low-intensity light (11 mW/mm(2)).