High-sensitive and high-efficient biochemical analysis method using a bionic electronic eye in combination with a smartphone-based colorimetric reader system
High-sensitive and high-efficient biochemical analysis method using a bionic electronic eye in combination with a smartphone-based colorimetric reader system
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DOI：
10.1016/j.snb.2015.04.052
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发表时间：
2015-09-01
影响因子：
8.4
通讯作者：
Wang, Ping
中科院分区：
文献类型：
--
作者：
Su, Kaiqi;Zou, Quchao;Wang, Ping
仿生电子眼（Bionic e-Eye）是一种基于智能手机开发的比色读取器系统，由智能手机或平板电脑（iPhone 4s或iPad 3）作为检测仪器和便携式配件作为照明提供者，集成广角镜头、一块最低功率的电致发光和定制的暗罩。将 96 孔微量滴定板 (MTP) 放置在电致发光装置上，Bionice Eye 通过智能手机的后置摄像头捕获检测图像。仿生电子眼的图像处理算法采用与人类视觉系统类似的色调、饱和度和明度（HSV，也称为六角锥模型）颜色模型。通过不同拍摄距离的系统稳定性实验确定了优化的系统尺寸。此外，还进行了市售的二辛可宁酸（BCA）蛋白测定和细胞计数试剂盒（CCK8）测定来评估这种仿生电子眼。对于这两种检测，Bionic e-Eye 的分析性能比微量滴定板读数器 (MTPR) 和之前基于智能手机的比色读数器具有更好的精度和更高的灵敏度。另外，采用光学图像检测的Bionic e-Eye具有同时和同步工作模式，而采用机器移动检测的MPTR在高吞吐量检测中具有异步工作模式。因此，仿生e-Eye将成为临床应用、工业质量控制、环境监测和食品评估领域理想的即时（POC）比色检测设备。 (C) 2015 Elsevier B.V. 保留所有权利。
Bionic electronic eye (Bionic e-Eye), a developed smartphone-based colorimetric reader system, consists of smartphone or pad (iPhone 4s or iPad 3) as detection instrument and portable accessory as illumination provider integrating with a wide-angle lens, a piece of lowest-power electro luminescent and a custom-made dark hood. A 96-well microtiter plate (MTP) was positioned on the electro luminescent and Bionice Eye captures the detection images by the back camera of smartphone. Being similar to human visual system, the hue, saturation and value (HSV, also called hex cone model) color model was employed in image processing algorithm of Bionic e-Eye. Optimized system dimension was determined by the system steadiness experiment of different photograph distances. Moreover, the commercially available bicinchoninic acid (BCA) protein assay and cell counting kit (CCK8) assay were carried out to evaluate this Bionic e-Eye. Analytical performance of Bionic e-Eye had the better precision, higher sensitivity than microtiter plate reader (MTPR) and previous smartphone-based colorimetric reader for both two assays. Also, Bionic e-Eye using optical image detection had simultaneous and synchronous working mode, while MPTR using machine moving detection had asynchronous working mode in high throughput detection. Therefore, Bionic e-Eye will be an ideal point-of-care (POC) colorimetric detection device in the field of clinical application, industrial quality control, environment monitoring, and food assessment. (C) 2015 Elsevier B.V. All rights reserved.